ArticleThe Journal of biological chemistry2016
Cloning of Glycerophosphocholine Acyltransferase (GPCAT) from Fungi and Plants: A NOVEL ENZYME IN PHOSPHATIDYLCHOLINE SYNTHESIS.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- The Batten disease protein CLN8 is a stereospecific acyltransferase in bis(monoacylglycero)phosphate biosynthesis.Nature cell biology · 2026Article
- Functional Prediction ofMembranes · 2026Article
- Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp.Microbial cell factories · 2025Article
- Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.Plant physiology · 2025Review
- Phosphatidylcholine biosynthesis pathways inFrontiers in cellular and infection microbiology · 2025Article
- Glycerophosphocholine provision rescuesmSphere · 2023Article
- TMEM164 is an acyltransferase that forms ferroptotic C20:4 ether phospholipids.Nature chemical biology · 2023Article
- Do betaine lipids replace phosphatidylcholine as fatty acid editing hubs in microalgae?Frontiers in plant science · 2023Review
- Phosphatidylcholine:diacylglycerol cholinephosphotransferase's unique regulation of castor bean oil quality.Plant physiology · 2022Article
- Enhanced production of hydroxy fatty acids in Arabidopsis seed through modification of multiple gene expression.Biotechnology for biofuels and bioproducts · 2022Article
- Diversity of ESI-MS Based Phosphatidylcholine Profiles in Basidiomycetes.Journal of fungi (Basel, Switzerland) · 2022Article
- Oil-Producing Metabolons Containing DGAT1 Use Separate Substrate Pools from those Containing DGAT2 or PDAT.Plant physiology · 2020Article
- Phospholipid turnover and acyl chain remodeling in the yeast ER.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Phosphatidylcholine Biosynthesis in Mitis Group Streptococci via Host Metabolite Scavenging.Journal of bacteriology · 2019Article
- Metabolically Distinct Pools of Phosphatidylcholine Are Involved in Trafficking of Fatty Acids out of and into the Chloroplast for Membrane Production.The Plant cell · 2019Article
- The glycerophosphocholine acyltransferase Gpc1 is part of a phosphatidylcholine (PC)-remodeling pathway that alters PC species in yeast.The Journal of biological chemistry · 2019Article
- The role of phospholipid molecular species in determining the physical properties of yeast membranes.FEBS letters · 2018Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Glycero-3-phosphocholine (GPC), the product of the complete deacylation of phosphatidylcholine (PC), was long thought to not be a substrate for reacylation. However, it was recently shown that cell-free extracts from yeast and plants could acylate GPC with acyl groups from acyl-CoA. By screening enzyme activities of extracts derived from a yeast knock-out collection, we were able to identify and clone the yeast gene (GPC1) encoding the enzyme, named glycerophosphocholine acyltransferase (GPCAT). By homology search, we also identified and cloned GPCAT genes from three plant species. All enzymes utilize acyl-CoA to acylate GPC, forming lyso-PC, and they show broad acyl specificities in both yeast and plants. In addition to acyl-CoA, GPCAT efficiently utilizes LPC and lysophosphatidylethanolamine as acyl donors in the acylation of GPC. GPCAT homologues were found in the major eukaryotic organism groups but not in prokaryotes or chordates. The enzyme forms its own protein family and does not contain any of the acyl binding or lipase motifs that are present in other studied acyltransferases and transacylases. In vivo labeling studies confirm a role for Gpc1p in PC biosynthesis in yeast. It is postulated that GPCATs contribute to the maintenance of PC homeostasis and also have specific functions in acyl editing of PC (e.g. in transferring acyl groups modified at the sn-2 position of PC to the sn-1 position of this molecule in plant cells).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.