Evidence map›Paper›PMID 27757798›Full record

ArticleMolecular biotechnology2016

Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants.

Aneela Yasmeen, Sarfraz Kiani, Afshan Butt, Abdul Qayyum Rao, Faheem Akram, Aftab Ahmad, Idrees Ahmad Nasir, Tayyab Husnain, Shahid Mansoor, Imran Amin and 6 more

Open access · hybridAbstract read
In one paragraph

Article in Molecular biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Aneela YasmeenCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Sarfraz KianiCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Afshan ButtCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Abdul Qayyum RaoCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan. qayyumabdul77@yahoo.com.
Faheem AkramCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Aftab AhmadCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Idrees Ahmad NasirCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Tayyab HusnainCenter of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Shahid MansoorNational Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Imran AminNational Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Shaheen AftabNational Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Muhammad ZubairNational Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Muhammad Nouman TahirNational Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Sohail AkhtarNational Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Jodi SchefflerJamie Whitten Delta States Research Center, USDA, Stoneville, MS, 38776, USA.
Brian SchefflerJamie Whitten Delta States Research Center, USDA, Stoneville, MS, 38776, USA.
National Institute for Biotechnology and Genetic Engineering · PKCentre of Excellence in Molecular Biology · PKUniversity of the Punjab · PKDelta Air Lines (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct targeting V2 gene of CLCuKoV-Bur, cloned under 35S promoter, was done in two elite cotton varieties MNH-786 and VH-289 using shoot apex cut method of gene transformation. The transformation efficiency was found to be 3.75 and 2.88 % in MNH-786 and VH-289, respectively. Confirmation of successful transformation was done through PCR in T

Indexed as

RNA InterferenceBegomovirusCapsid ProteinsChromosomes, PlantDisease ResistanceGene TargetingGossypiumPlant DiseasesPlants, Genetically ModifiedTransgenesViral LoadVirus ReplicationCapsid ProteinsBegomovirusCLCuDGene transformationKnockdownsiRNAV2 ORF

Identifiers

PMID27757798
PMCPMC5102983
OpenAlexW2535797044

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.