ArticleExtremophiles : life under extreme conditions2017
Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11.
Article in Extremophiles : life under extreme conditions, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Site-specific gene integration by recombinase-mediated cassette exchange in anhydrobiotic Pv11 cells.In vitro cellular & developmental biology. Animal · 2026Article
- An anhydrobiotic cell line expressing odorant receptors shows odorant responses after dry storage.Scientific reports · 2025Article
- A sodium-dependent trehalose transporter contributes to anhydrobiosis in insect cell line, Pv11.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Effective methods for immobilization of non-adherent Pv11 cells while maintaining their desiccation tolerance.Cytotechnology · 2023Article
- Deciphering the Biological Enigma-Genomic Evolution Underlying Anhydrobiosis in the Phylum Tardigrada and the ChironomidInsects · 2022Review
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- Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells.Scientific reports · 2021Article
- Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11.International journal of molecular sciences · 2021Article
- Development of a Tet-On Inducible Expression System for the Anhydrobiotic Cell Line, Pv11.Insects · 2020Article
- New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki.Scientific reports · 2020Article
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- Identification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines.Scientific reports · 2019Article
- Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomidProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Towards water-free biobanks: long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells.Scientific reports · 2017Article
- International Conference on Extremophiles 2016.Extremophiles : life under extreme conditions · 2017Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Larvae of the African midge Polypedilum vanderplanki show extreme desiccation tolerance, known as anhydrobiosis. Recently, the cultured cell line Pv11 was derived from this species; Pv11 cells can be preserved in the dry state for over 6 months and retain their proliferation potential. Here, we attempted to expand the use of Pv11 cells as a model to investigate the mechanisms underlying anhydrobiosis in P. vanderplanki. A newly developed vector comprising a constitutive promoter for the PvGapdh gene allowed the expression of exogenous proteins in Pv11 cells. Using this vector, a stable Pv11 cell line expressing green fluorescence protein (GFP) was established and retained desiccation tolerance. Gene silencing with GFP-specific siRNAs significantly suppressed GFP expression to approximately 7.5-34.6% of that in the non-siRNA-transfected GFP stable line. Establishment of these functional assays will enable Pv11 cells to be utilized as an effective tool to investigate the molecular mechanisms underlying anhydrobiosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.