ArticleJournal of industrial microbiology & biotechnology2016
FkbN and Tcs7 are pathway-specific regulators of the FK506 biosynthetic gene cluster in Streptomyces tsukubaensis L19.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 29 citations in OpenAlex.
- Enhancement of tacrolimus production in Streptomyces tsukubaensis by genetic engineering and process optimization.World journal of microbiology & biotechnology · 2025Article
- Enhancement of spinosad production in Saccharopolyspora spinosa by overexpression of the complete 74-kb spinosyn gene cluster.Microbial cell factories · 2025Article
- Stepwise increase of fidaxomicin in an engineered heterologous hostSynthetic and systems biotechnology · 2024Article
- The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis inSynthetic and systems biotechnology · 2024Article
- Enhancing tylosin production by combinatorial overexpression of efflux, SAM biosynthesis, and regulatory genes in hyperproducingSynthetic and systems biotechnology · 2023Article
- Improvement of FK506 production via metabolic engineering-guided combinational strategies in Streptomyces tsukubaensis.Microbial cell factories · 2021Article
- Activation and discovery of tsukubarubicin from Streptomyces tsukubaensis through overexpressing SARPs.Applied microbiology and biotechnology · 2021Article
- The Onset of Tacrolimus Biosynthesis inAntibiotics (Basel, Switzerland) · 2020Article
- Crotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus.Communications biology · 2020Article
- The Application of Regulatory Cascades inFrontiers in microbiology · 2020Review
- Article
- Unraveling Nutritional Regulation of Tacrolimus Biosynthesis inAntibiotics (Basel, Switzerland) · 2018Review
- Combining metabolomics and network analysis to improve tacrolimus production in Streptomyces tsukubaensis using different exogenous feedings.Journal of industrial microbiology & biotechnology · 2017Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
FK506 (tacrolimus), which is produced by many Streptomyces strains, is clinically used as an immunosuppressive agent and for treatment of inflammatory skin diseases. Here, we identified that the FK506 biosynthetic gene cluster in an industrial FK506-producing strain Streptomyces tsukubaensis L19 is organized as eight transcription units. Two pathway-specific regulators, FkbN and Tcs7, involved in FK506 biosynthesis from S. tsukubaensis L19 were characterized in vivo and in vitro. FkbN activates the transcription of six transcription units in FK506 biosynthetic gene cluster, and Tcs7 activates the transcription of fkbN. In addition, the DNA-binding specificity of FkbN was determined. Finally, a high FK506-producing strain was constructed by overexpression of both fkbN and tcs7 in S. tsukubaensis L19, which improved FK506 production by 89 % compared to the parental strain.
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