Evidence map›Paper›PMID 27757329›Full record

ArticlePlastic and reconstructive surgery. Global open2016

Mechanical Stimulation Increases Knee Meniscus Gene RNA-level Expression in Adipose-derived Stromal Cells.

Elizabeth M Meier, Bin Wu, Aamir Siddiqui, Donna G Tepper, Michael T Longaker, Mai T Lam

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plastic and reconstructive surgery. Global open, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Elizabeth M MeierDepartment of Biomedical Engineering, Wayne State University, Detroit, Mich.; Department of Plastic Surgery, Henry Ford Health System, Detroit, Mich.; and Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.
Bin WuDepartment of Biomedical Engineering, Wayne State University, Detroit, Mich.; Department of Plastic Surgery, Henry Ford Health System, Detroit, Mich.; and Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.
Aamir SiddiquiDepartment of Biomedical Engineering, Wayne State University, Detroit, Mich.; Department of Plastic Surgery, Henry Ford Health System, Detroit, Mich.; and Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.
Donna G TepperDepartment of Biomedical Engineering, Wayne State University, Detroit, Mich.; Department of Plastic Surgery, Henry Ford Health System, Detroit, Mich.; and Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.
Michael T LongakerDepartment of Biomedical Engineering, Wayne State University, Detroit, Mich.; Department of Plastic Surgery, Henry Ford Health System, Detroit, Mich.; and Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.
Mai T LamDepartment of Biomedical Engineering, Wayne State University, Detroit, Mich.; Department of Plastic Surgery, Henry Ford Health System, Detroit, Mich.; and Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.
Wayne State University · US

Funding

Induced pluripotent stem cells in the understanding and treatment of heart diseaseU01HL099776 · NHLBI · STANFORD UNIVERSITY · PI LONGAKER, MICHAEL T, WU, JOSEPH C. · 2009 to 2015
$8.2M
Osteogenic Enrichment of Adipose Derived Stromal CellsR01DE021683 · NIDCR · STANFORD UNIVERSITY · PI GURTNER, GEOFFREY C, LONGAKER, MICHAEL T · 2012 to 2016
$2.0M
Regional differences in neural crest and mesodermal derived calvarial bone healinR01DE019434 · NIDCR · STANFORD UNIVERSITY · PI LONGAKER, MICHAEL T · 2010 to 2013
$1.5M
NHLBI NIH HHS U01 HL099776NIDCR NIH HHS R01 DE019434NIDCR NIH HHS R01 DE021683
6 · The paper itself

Abstract

Efforts have been made to engineer knee meniscus tissue for injury repair, yet most attempts have been unsuccessful. Creating a cell source that resembles the complex, heterogeneous phenotype of the meniscus cell remains difficult. Stem cell differentiation has been investigated, mainly using bone marrow mesenchymal cells and biochemical means for differentiation, resulting in no solution. Mechanical stimulation has been investigated to an extent with no conclusion. Here, we explore the potential for and effectiveness of mechanical stimulation to induce the meniscal phenotype in adipose-derived stromal cells.

methodsHuman adipose-derived stromal cells were chosen for their fibrogenic nature and conduciveness for chondrogenesis. Biochemical and mechanical stimulation were investigated. Biochemical stimulation included fibrogenic and chondrogenic media. For mechanical stimulation, a custom-built device was used to apply constant, cyclical, uniaxial strain for up to 6 hours. Strain and frequency varied.

resultsUnder biochemical stimulation, both fibrogenic (collagen I, versican) and chondrogenic (collagen II, Sox9, aggrecan) genes were expressed by cells exposed to either fibrogenic or chondrogenic biochemical factors. Mechanical strain was found to preferentially promote fibrogenesis over chondrogenesis, confirming that tensile strain is an effective fibrogenic cue. Three hours at 10% strain and 1 Hz in chondrogenic media resulted in the highest expression of fibrochondrogenic genes. Although mechanical stimulation did not seem to affect protein level expression, biochemical means did affect protein level presence of collagen fibers.

conclusionMechanical stimulation can be a useful differentiation tool for mechanoresponsive cell types as long as biochemical factors are also integrated.

Identifiers

PMID27757329
PMCPMC5054995
OpenAlexW2520489345

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.