ArticleScientific reports2016
Discovery of putative capsaicin biosynthetic genes by RNA-Seq and digital gene expression analysis of pepper.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Light regulates capsaicinoid biosynthesis via the CaHY5-CaBBX2-CaACS8 module in pepper.The New phytologist · 2026Article
- Next-Generation Metabolic Engineering of Capsaicinoids Biosynthesis in Chilli Pepper: Bridging Genomic Insights to Biotechnological Applications.Biotech (Basel (Switzerland)) · 2026Review
- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- Genetic and epigenetic mechanisms underlying cadmium (Cd) stress responses in soybean cultivars.Plant cell reports · 2026Article
- A comprehensive review of capsaicin: Biosynthesis, industrial productions, processing to applications, and clinical uses.Heliyon · 2024Review
- Unlocking the potential of capsaicin in oral health (Review).Biomedical reports · 2024Review
- Full-length transcriptome sequencing of pepper fruit during development and construction of a transcript variation database.Horticulture research · 2024Article
- Two telomere-to-telomere gapless genomes reveal insights into Capsicum evolution and capsaicinoid biosynthesis.Nature communications · 2024Article
- Capsaicinoids and Their Effects on Cancer: The "Double-Edged Sword" Postulate from the Molecular Scale.Cells · 2023Review
- Improved antioxidant activities of spice require enrichment of distinct yet closely-related metabolic pathways.Heliyon · 2023Article
- Genome-Wide Identification ofInternational journal of molecular sciences · 2023Article
- Post-Harvest Red- and Far-Red-Light Irradiation and Low Temperature Induce the Accumulation of Carotenoids, Capsaicinoids, and Ascorbic Acid inFoods (Basel, Switzerland) · 2023Article
- MYB24 Negatively Regulates the Biosynthesis of Lignin and Capsaicin by Affecting the Expression of Key Genes in the Phenylpropanoid Metabolism Pathway inMolecules (Basel, Switzerland) · 2023Article
- Lipoxygenase (LOX) in Sweet and Hot Pepper (International journal of molecular sciences · 2022Article
- Transcriptional Regulation of Ripening in Chili Pepper Fruits (International journal of molecular sciences · 2021Review
- Capsaicinoid biosynthesis in the pericarp of chili pepper fruits is associated with a placental septum-like transcriptome profile and tissue structure.Plant cell reports · 2021Article
- Attaining the promise of plant gene editing at scale.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- Transcriptome profiling of Capsicum annuum using Illumina- and PacBio SMRT-based RNA-Seq for in-depth understanding of genes involved in trichome formation.Scientific reports · 2021Article
- Three Strains ofViruses · 2021Article
- Biochemistry and molecular biology of capsaicinoid biosynthesis: recent advances and perspectives.Plant cell reports · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Indian pepper 'Guijiangwang' (Capsicum frutescens L.), one of the world's hottest chili peppers, is rich in capsaicinoids. The accumulation of the alkaloid capsaicin and its analogs in the epidermal cells of the placenta contribute to the pungency of Capsicum fruits. To identify putative genes involved in capsaicin biosynthesis, RNA-Seq was used to analyze the pepper's expression profiles over five developmental stages. Five cDNA libraries were constructed from the total RNA of placental tissue and sequenced using an Illumina HiSeq 2000. More than 19 million clean reads were obtained from each library, and greater than 50% of the reads were assignable to reference genes. Digital gene expression (DGE) profile analysis using Solexa sequencing was performed at five fruit developmental stages and resulted in the identification of 135 genes of known function; their expression patterns were compared to the capsaicin accumulation pattern. Ten genes of known function were identified as most likely to be involved in regulating capsaicin synthesis. Additionally, 20 new candidate genes were identified related to capsaicin synthesis. We use a combination of RNA-Seq and DGE analyses to contribute to the understanding of the biosynthetic regulatory mechanism(s) of secondary metabolites in a nonmodel plant and to identify candidate enzyme-encoding genes.
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