ArticleScientific reports2016
Synchronized human skeletal myotubes of lean, obese and type 2 diabetic patients maintain circadian oscillation of clock genes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 51 citations in OpenAlex.
- The relationship between circadian type and physical activity as predictors of cognitive performance during simulated nightshifts: A randomised controlled trial.Chronobiology international · 2025Trial
- Stable clocks, rewired rhythms: circadian remodeling in skeletal muscle during aging, cachexia, and type 2 diabetes.Life metabolism · 2026Review
- Rev-erb-α in Skeletal Muscle Adaptation: Integrating Circadian Control Mechanisms and Exercise Dynamic Responses.Journal of cellular physiology · 2026Review
- Restoration of circadian rhythm as novel targets against sarcopenia.Chinese medical journal · 2026Review
- Circadian Regulation of Glucose Metabolism: Implications for Pathogenesis and Chronotherapy of Type 2 Diabetes.Diabetology · 2026Article
- Exercise Timing in Sport: Molecular and Physiological Mechanisms Linking Performance, Recovery, and Biological Cost.International journal of molecular sciences · 2026Review
- Live-cell GLUT4 translocation assay reveals Per3 as a novel regulator of circadian insulin sensitivity in skeletal muscle cells.Biology open · 2025Article
- Physiological rhythms and metabolic regulation: Shining light on skeletal muscle.Experimental physiology · 2025Review
- The Effect of Sleep Disruption on Cardiometabolic Health.Life (Basel, Switzerland) · 2025Review
- Clockwork conditioning: Aligning the skeletal muscle clock with time-of-day exercise for cardiometabolic health.Journal of molecular and cellular cardiology · 2025Review
- More than the clock: distinct regulation of muscle function and metabolism by PER2 and RORα.The Journal of physiology · 2024Article
- Move the night way: how can physical activity facilitate adaptation to shift work?Communications biology · 2024Review
- Effects of resveratrol onAging · 2024Article
- In Vitro Circadian Clock Gene Expression Assessments in Mesenchymal Stem Cells from Human Infants: A Pilot Study.Nutrients · 2023Article
- Resveratrol as a potential protective compound against skeletal muscle insulin resistance.Heliyon · 2023Review
- Effects of Diet and Exercise on Circadian Rhythm: Role of Gut Microbiota in Immune and Metabolic Systems.Nutrients · 2023Review
- Metabolism and exercise: the skeletal muscle clock takes centre stage.Nature reviews. Endocrinology · 2023Review
- Time for Exercise? Exercise and Its Influence on the Skeletal Muscle Clock.Journal of biological rhythms · 2022Article
- Disrupted circadian oscillations in type 2 diabetes are linked to altered rhythmic mitochondrial metabolism in skeletal muscle.Science advances · 2021Article
- Atomoxetine and circadian gene expression in human dermal fibroblasts from study participants with a diagnosis of attention-deficit hyperactivity disorder.Journal of neural transmission (Vienna, Austria : 1996) · 2021Article
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell and animal studies have demonstrated that circadian rhythm is governed by autonomous rhythmicity of clock genes. Although disturbances in circadian rhythm have been implicated in metabolic disease development, it remains unknown whether muscle circadian rhythm is altered in human models of type 2 diabetes. Here we used human primary myotubes (HPM) to investigate if rhythmicity of clock- and metabolic gene expression is altered in donors with obesity or type 2 diabetes compared to metabolically healthy donors. HPM were obtained from skeletal muscle biopsies of four groups: type 2 diabetic patients and their BMI- and age-matched obese controls and from lean, healthy and young endurance trained athletes and their age-matched sedentary controls. HPM were differentiated for 7 days before synchronization by serum shock followed by gene expression profiling over the next 72 hours. HPM display robust circadian rhythms in clock genes, but REVERBA displayed dampened rhythmicity in type 2 diabetes. Furthermore, rhythmicity in NAMPT and SIRT1 expression was only observed in HPM from trained athletes. Rhythmicity in expression of key-regulators of carbohydrate and lipid metabolism was modest. We demonstrate that in human skeletal muscle REVERBA/B, NAMPT and SIRT1 circadian rhythms are affected in donors of sedentary life style and poor health status.
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