ArticlePlant physiology2016
Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
- Overview of oxidative metabolism and autophagy under metal stress.Journal of experimental botany · 2026Review
- Hydrogen peroxide as a multifaceted regulator of the Atg4 protease and autophagy in the pathogenic fungusFrontiers in fungal biology · 2026Article
- Evolutionary conservation and metabolic significance of autophagy in algae.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Autophagy in plants.Autophagy reports · 2024Review
- Mechanisms controlling plant proteases and their substrates.Cell death and differentiation · 2023Review
- Whole-Genome Sequencing and Transcriptome Analysis ofJournal of fungi (Basel, Switzerland) · 2022Article
- When the Phagosome Gets Leaky: Pore-Forming Toxin-Induced Non-Canonical Autophagy (PINCA).Frontiers in cellular and infection microbiology · 2022Article
- Canonical and Non-Canonical Functions of the Autophagy Machinery in MHC Restricted Antigen Presentation.Frontiers in immunology · 2022Review
- Monitoring of ATG4 Protease Activity During Autophagy in the Model Microalga Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Chlamydomonas proteases: classification, phylogeny, and molecular mechanisms.Journal of experimental botany · 2021Article
- Autophagy Is Involved in the Viability of Overexpressing ThioredoxinAntioxidants (Basel, Switzerland) · 2021Article
- Persulfidation of ATG18a regulates autophagy under ER stress inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Oxidation inhibits autophagy protein deconjugation from phagosomes to sustain MHC class II restricted antigen presentation.Nature communications · 2021Article
- Autophagy Induced Accumulation of Lipids inFrontiers in plant science · 2021Article
- Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide.The Plant cell · 2020Article
- Are Flavonoids Effective Antioxidants in Plants? Twenty Years of Our Investigation.Antioxidants (Basel, Switzerland) · 2020Review
- The protease activity of human ATG4B is regulated by reversible oxidative modification.Autophagy · 2020Article
- Redox-Mediated Post-Translational Modifications of Proteolytic Enzymes and Their Role in Protease Functioning.Biomolecules · 2020Review
- The MicroalgaPlant physiology · 2020Article
- Knowing When to Self-Eat - Fine-Tuning Autophagy Through ATG8 Iso-forms in Plants.Frontiers in plant science · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is a major catabolic pathway by which eukaryotic cells deliver unnecessary or damaged cytoplasmic material to the vacuole for its degradation and recycling in order to maintain cellular homeostasis. Control of autophagy has been associated with the production of reactive oxygen species in several organisms, including plants and algae, but the precise regulatory molecular mechanisms remain unclear. Here, we show that the ATG4 protease, an essential protein for autophagosome biogenesis, plays a central role for the redox regulation of autophagy in the model green alga Chlamydomonas reinhardtii Our results indicate that the activity of C. reinhardtii ATG4 is regulated by the formation of a single disulfide bond with a low redox potential that can be efficiently reduced by the NADPH/thioredoxin system. Moreover, we found that treatment of C. reinhardtii cells with norflurazon, an inhibitor of carotenoid biosynthesis that generates reactive oxygen species and triggers autophagy in this alga, promotes the oxidation and aggregation of ATG4. We propose that the activity of the ATG4 protease is finely regulated by the intracellular redox state, and it is inhibited under stress conditions to ensure lipidation of ATG8 and thus autophagy progression in C. reinhardtii.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.