ArticleBMC research notes2016
Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study.
Article in BMC research notes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 42 citations in OpenAlex.
- Genomic landscape ofApplied and environmental microbiology · 2026Article
- A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries.Microorganisms · 2025Article
- Genomic characterization ofFrontiers in microbiology · 2025Article
- Mitigating antimicrobial resistance through effective hospital wastewater management in low- and middle-income countries.Frontiers in public health · 2024Article
- Vibrio cholerae O1 Inhabit Intestines and Spleens of Fish in Aquaculture Ponds.Microbial ecology · 2023Article
- Incidence and Virulence Factor Profiling ofMicroorganisms · 2023Article
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- SARS-CoV-2 Burden in Wastewater and its Elimination Using Disinfection.Microbiology insights · 2023Review
- Virulence genes and antibiotic resistance profile ofVeterinary research forum : an international quarterly journal · 2022Article
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- Utilization of SARS-CoV-2 Wastewater Surveillance in Africa-A Rapid Review.International journal of environmental research and public health · 2022Review
- Abundance and biofilm formation capability ofFrontiers in microbiology · 2022Article
- Coupled DNA-labeling and sequencing approach enables the detection of viable-but-non-culturable Vibrio spp. in irrigation water sources in the Chesapeake Bay watershed.Environmental microbiome · 2021Article
- Antimicrobial Blue Light versus Pathogenic Bacteria: Mechanism, Application in the Food Industry, Hurdle Technologies and Potential Resistance.Foods (Basel, Switzerland) · 2020Review
- Accumulating evidence suggests that some waterbird species are potential vectors of Vibrio cholerae.PLoS pathogens · 2019Review
- Review
- Tilapia (Frontiers in microbiology · 2019Article
- Surveillance and Genomics of ToxigenicFrontiers in microbiology · 2019Article
- The transcriptome analysis of the Arabidopsis thaliana in response to the Vibrio vulnificus by RNA-sequencing.PloS one · 2019Article
- Transmission and Toxigenic Potential ofFrontiers in microbiology · 2018Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCholera, one of the world's deadliest infectious diseases, remains rampant and frequent in Tanzania and thus hinders existing control measures. The present study was undertaken to evaluate the occurrence of toxigenic Vibrio cholerae O1 in wastewater, fish and vegetables during a non-outbreak period in Morogoro, Tanzania.
methodsFrom October 2014 to February 2015, 60 wastewater samples, 60 fish samples from sewage stabilization ponds and 60 wastewater irrigated vegetable samples were collected. Samples were cultured for identification of V. cholerae using conventional bacteriological methods. Isolates were confirmed as V. cholerae by detection of the outer membrane protein gene (ompW) using polymerase chain reaction (PCR). Isolates were further tested for antibiotic susceptibility and presence of virulence genes including, cholera enterotoxin gene (ctx), the toxin co-regulated pilus gene (tcpA) and the haemolysin gene (hlyA).
resultsThe prevalence of V. cholerae in wastewater, vegetables and fish was 36.7, 21.7 and 23.3 %, respectively. Two isolates from fish gills were V. cholerae O1 and tested positive for ctx and tcpA. One of these contained in addition the hlyA gene while five isolates from fish intestines tested positive for tcpA. All V. cholerae isolates were resistant to ampicillin, amoxicillin and some to tetracycline, but sensitive to gentamicin, chloramphenicol, and ciprofloxacin.
conclusionsOur results show that toxigenic and drug-resistant V. cholerae O1 species are present and persist in aquatic environments during a non-cholera outbreak period. This is of public health importance and shows that such environments may be important as reservoirs and in the transmission of V. cholerae O1.
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