ArticleJournal of the American Chemical Society2016
The N-Terminal Amphipathic Helix of Endophilin Does Not Contribute to Its Molecular Curvature Generation Capacity.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Recombinant protein platform for high-throughput investigation of peptide-liposome interactions via fluorescence anisotropy depolarization.Communications chemistry · 2026Article
- Bending the boundaries: the many facets of endophilin-As from membrane dynamics to disease.Cellular and molecular life sciences : CMLS · 2025Review
- GTP hydrolysis triggers membrane remodeling by AMPH-1.Science advances · 2025Article
- Biology of endophilin and it's role in disease.Frontiers in immunology · 2023Review
- Generation of nanoscopic membrane curvature for membrane trafficking.Nature reviews. Molecular cell biology · 2023Review
- Remodeling of the Plasma Membrane by Surface-Bound Protein Monomers and Oligomers: The Critical Role of Intrinsically Disordered Regions.The Journal of membrane biology · 2022Review
- Multivalent interactions between molecular components involved in fast endophilin mediated endocytosis drive protein phase separation.Nature communications · 2022Article
- Insights into Membrane Curvature Sensing and Membrane Remodeling by Intrinsically Disordered Proteins and Protein Regions.The Journal of membrane biology · 2022Review
- The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo.Developmental cell · 2022Article
- Membrane partitioning and lipid selectivity of the N-terminal amphipathic H0 helices of endophilin isoforms.Biochimica et biophysica acta. Biomembranes · 2021Article
- Unfolding Mechanisms and Conformational Stability of the Dimeric Endophilin N-BAR Domain.ACS omega · 2021Article
- FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria.PLoS biology · 2021Article
- Protein-dependent membrane remodeling in mitochondrial morphology and clathrin-mediated endocytosis.European biophysics journal : EBJ · 2021Review
- Cellular Blebs and Membrane Invaginations Are Coupled through Membrane Tension Buffering.Biophysical journal · 2019Article
- The plasma membrane as a mechanochemical transducer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Protein Amphipathic Helix Insertion: A Mechanism to Induce Membrane Fission.Frontiers in cell and developmental biology · 2019Review
- Simulations of N-BAR Protein Interactions with Membranes.Journal of physics D: Applied physics · 2018Article
- Structure Versus Stochasticity-The Role of Molecular Crowding and Intrinsic Disorder in Membrane Fission.Journal of molecular biology · 2018Review
- The 2018 biomembrane curvature and remodeling roadmap.Journal of physics D: Applied physics · 2018Article
- Organizing membrane-curving proteins: the emerging dynamical picture.Current opinion in structural biology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
N-BAR proteins such as endophilin are thought to bend lipid membranes via scaffolding (the molding of membranes through the crescent protein shape) and membrane insertion (also called wedging) of amphipathic helices. However, the contributions from these distinct mechanisms to membrane curvature generation and sensing have remained controversial. Here we quantitatively demonstrate that the amphipathic N-terminal H0 helix of endophilin is important for recruiting this protein to the membrane, but does not contribute significantly to its intrinsic membrane curvature generation capacity. These observations elevate the importance of the scaffolding mechanism, rather than H0 insertion, for the membrane curvature generation by N-BAR domains. Furthermore, consistent with the thermodynamically required coupling between curvature generation and sensing, we observed that the H0-truncated N-BAR domain is capable of sensing membrane curvature. Overall, our contribution clarifies an important mechanistic controversy in the function of N-BAR domain proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.