ArticleHypertension (Dallas, Tex. : 1979)2016
Hypomethylation of CYP11B2 in Aldosterone-Producing Adenoma.
Article in Hypertension (Dallas, Tex. : 1979), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Inter- and intratumoral heterogeneity of aldosterone-producing adenomas: recent advances and clinical implications.Endocrine journal · 2026Review
- Age-varying DNA methylation patterns associated with blood pressure in mid-to-late adulthood.Clinical epigenetics · 2026Article
- Age-varying DNA methylation patterns associated with blood pressure across the lifespan.bioRxiv : the preprint server for biology · 2025Article
- Adrenocortical Tumor Associated With Pathogenic Variant in KCNJ5 and DNA Methylation of CYP11B2 in Primary Aldosteronism.JCEM case reports · 2024Article
- Molecular and Epigenetic Control of Aldosterone Synthase, CYP11B2 and 11-Hydroxylase, CYP11B1.International journal of molecular sciences · 2023Review
- Association of DNA methylation with steroidogenic enzymes in Cushing's adenoma.Endocrine-related cancer · 2022Article
- Hypomethylation associated vitamin D receptor expression in ATP1A1 mutant aldosterone-producing adenoma.Molecular and cellular endocrinology · 2022Article
- Primary aldosteronism: Pathophysiological mechanisms of cell death and proliferation.Frontiers in endocrinology · 2022Review
- Review
- DNA Methylation of the Angiotensinogen Gene,International journal of molecular sciences · 2021Review
- Update on Genetics of Primary Aldosteronism.Biomedicines · 2021Review
- The landscape of molecular mechanism for aldosterone production in aldosterone-producing adenoma.Endocrine journal · 2020Review
- Molecular Basis of Primary Aldosteronism and Adrenal Cushing Syndrome.Journal of the Endocrine Society · 2020Review
- Endoplasmic Reticulum Chaperone Calmegin Is Upregulated in Aldosterone-Producing Adenoma and Associates With Aldosterone Production.Hypertension (Dallas, Tex. : 1979) · 2020Article
- Genetic causes of primary aldosteronism.Experimental & molecular medicine · 2019Review
- The Biology of Normal Zona Glomerulosa and Aldosterone-Producing Adenoma: Pathological Implications.Endocrine reviews · 2018Review
- Epigenetic Regulation of Aldosterone Synthase Gene by Sodium and Angiotensin II.Journal of the American Heart Association · 2018Article
- MiR-193a-3p functions as a tumour suppressor in human aldosterone-producing adrenocortical adenoma by down-regulating CYP11B2.International journal of experimental pathology · 2018Article
- Calneuron 1 Increased CaHypertension (Dallas, Tex. : 1979) · 2018Article
- The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion.JCI insight · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The purpose of this study was to evaluate the DNA methylation levels of steroidogenic enzyme genes in aldosterone-producing adenoma (APA) and the effects of gene mutations in APA on the DNA methylation levels. DNA methylation array analysis was conducted using nonfunctioning adrenocortical adenoma (n=12) and APA (n=35) samples, including some with a KCNJ5 mutation (n=21), an ATP1A1 mutation (n=5), and without the known mutations (n=9). The quantitative polymerase chain reaction assay was performed for the detection of CYP11B2 and CYP11B1 expression levels in nonfunctioning adrenocortical adenoma and APA. We introduced the KCNJ5 T158A mutation using lentivirus delivery in the human adrenocortical 15 cell line, and analyzed the effects of the mutation on DNA methylation levels. We analyzed the 83 presumed DNA methylation sites of steroidogenic enzymes. In APA, we found 7 hypomethylated sites in CYP11B2 and 1 hypomethylated and 6 hypermethylated sites in CYP11B1 There were no differences in the steroidogenic enzymes gene DNA methylation of peripheral leukocytes between nonfunctioning adrenocortical adenoma and APA. No CYP11B2 methylation level was associated with CYP11B2 transcription levels in APA. All methylation sites, except for a CYP11B2 region, showed no difference among APAs with or without gene mutations. Human adrenocortical 15 cells with the KCNJ5 mutation showed no changes in CYP11B2 or CYP11B1 methylation levels compared with control cells. We demonstrated that CYP11B2 in APA was extensively hypomethylated, and CYP11B2 methylation in the region with hypomethylation was not induced by KCNJ5 or ATP1A1 mutations that cause aldosterone overproduction in APA and a KCNJ5 mutation human adrenocortical 15 cells.
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