ArticleInternational journal of molecular sciences2016
Development of an Advanced HPLC-MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Criterion-Related Validity of Spectroscopy-Based Skin Carotenoid Measurements as a Proxy for Fruit and Vegetable Intake: A Systematic Review.Advances in nutrition (Bethesda, Md.) · 2020Pooled it
- Carotenoids in the Context of the Mediterranean Dietary Pattern: From Food Matrix and Bioavailability to Biomarkers and Human Health.Antioxidants (Basel, Switzerland) · 2026Review
- Rationale and study protocol for a randomized controlled MIND diet feeding trial for breast cancer-related cognitive impairment.Contemporary clinical trials · 2026Article
- Serum and Skin Carotenoid Levels in Older Adults with and Without Metabolic Syndrome: A Cross-Sectional Study.Nutrients · 2025Article
- Recent advances in electrochemical sensors for vitamin sensing: toward point-of-care micronutrient assessment.Discover nano · 2025Review
- Development of low cost ZnO based chemi resistive biosensor for the detection of vitamin B6 mediated by quantum dots.Scientific reports · 2025Article
- Rapid Determination of Water-Soluble Vitamins in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.ACS omega · 2025Article
- Review
- Article
- Development of an HPLC-PDA Method for the Determination of Capsanthin, Zeaxanthin, Lutein, β-Cryptoxanthin and β-Carotene Simultaneously in Chili Peppers and Products.Molecules (Basel, Switzerland) · 2023Article
- Factors determining the oral absorption and systemic disposition of zeaxanthin in rats:Pharmaceutical biology · 2022Article
- Integrated Metabolomics, Lipidomics, and Genomics Reveal the Presence of a New Biomarker, Butanediol Glucuronide, Associated with the Activation of Liver Ketogenesis and Lipid Oxidation by Tomato-BasedAntioxidants (Basel, Switzerland) · 2022Article
- Diversity and Content of Carotenoids and Other Pigments in the Transition from the Green to the Red Stage ofPlants (Basel, Switzerland) · 2022Article
- Circulating carotenoids are associated with favorable lipid and fatty acid profiles in an older population at high cardiovascular risk.Frontiers in nutrition · 2022Article
- NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial.Frontiers in nutrition · 2022Article
- Identification and Content of Astaxanthin and Its Esters from MicroalgaePlants (Basel, Switzerland) · 2021Article
- Comparative Study for the Determination of Fat-Soluble Vitamins in Rice Cereal Baby Foods Using HPLC-DAD and UHPLC-APCI-MS/MS.Foods (Basel, Switzerland) · 2021Article
- High Fruit and Vegetable Consumption and Moderate Fat Intake Are Associated with Higher Carotenoid Concentration in Human Plasma.Antioxidants (Basel, Switzerland) · 2021Article
- Using Extra Virgin Olive Oil to Cook Vegetables Enhances Polyphenol and Carotenoid Extractability: A Study Applying theMolecules (Basel, Switzerland) · 2019Article
- New LC-MS/MS method with single-step pretreatment analyzes fat-soluble vitamins in plasma and amniotic fluid.Journal of lipid research · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC-MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC-DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids.
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Registered trials
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