ArticleJournal of biophotonics2017
Intravital excitation increases detection sensitivity for pulmonary tuberculosis by whole-body imaging with β-lactamase reporter enzyme fluorescence.
Article in Journal of biophotonics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Review
- Highly Sensitive In Vivo Imaging of Bacterial Infections with a Hydrophilicity-Switching, Self-Immobilizing, Near-Infrared Fluorogenic β-Lactamase Probe Enriched within Bacteria.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fluorescence modeling ofBiomedical optics express · 2019Article
- Monitoring Tuberculosis Drug Activity in Live Animals by Near-Infrared Fluorescence Imaging.Antimicrobial agents and chemotherapy · 2019Article
- Light scattering by pulmonary alveoli and airway surface liquid using a concentric sphere model.Optics letters · 2018Article
- Optical model of the murine lung to optimize pulmonary illumination.Journal of biomedical optics · 2018Article
- Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence.Journal of visualized experiments : JoVE · 2018Article
- Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure.Journal of visualized experiments : JoVE · 2018Article
- Formulation of image fusion as a constrained least squares optimization problem.Journal of medical imaging (Bellingham, Wash.) · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Tuberculosis is a pulmonary disease with an especially high mortality rate in immuno-compromised populations, specifically children and HIV positive individuals. The causative agent, Mycobacterium tuberculosis (Mtb), is a very slow growing and difficult organism to work with, making both diagnosis and development of effective treatments cumbersome. We utilize a fiber-optic fluorescence microendoscope integrated with a whole-body imaging system for in vivo Mtb detection. The system exploits an endogenous enzyme of Mtb (β-lactamase, or BlaC) using a BlaC-specific NIR fluorogenic substrate. In the presence of BlaC, this substrate is cleaved and becomes fluorescent. Using intravital illumination of the lung to excite this probe, sensitivity of the optical system increases over trans- and epi-illumination methods of whole-body fluorescence imaging. We demonstrate that integration of these imaging technologies with BlaC-specific fluorescent reporter probe improves the level of detection to ∼100 colony forming units, a 100× increase in sensitivity in comparison to epi-illumination and a 10× increase in sensitivity in comparison to previous work in intravital excitation of tdTomato-expressing Mtb. This lower detection threshold enables the study of early stage bacterial infections with clinical strains of Mtb and longitudinal studies of disease pathogenesis and therapeutic efficacy with multiple time points in a single animal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.