ArticleScientific reports2016
DNA damage precedes apoptosis during the regression of the interdigital tissue in vertebrate embryos.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Lysosomes, caspase-mediated apoptosis, and cytoplasmic activation of P21, but not cell senescence, participate in a redundant fashion in embryonic morphogenetic cell death.Cell death & disease · 2023Article
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- Regulation of Developmental Cell Death in the Animal Kingdom: A Critical Analysis of Epigenetic versus Genetic Factors.International journal of molecular sciences · 2022Review
- High Levels of CD244 Rather Than CD160 Associate With CD8Frontiers in immunology · 2022Article
- Cell death in the developing vertebrate limb: A locally regulated mechanism contributing to musculoskeletal tissue morphogenesis and differentiation.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
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- Is Senescence-Associated β-Galactosidase a ReliableFrontiers in cell and developmental biology · 2021Review
- The methylation status of the embryonic limb skeletal progenitors determines their cell fate in chicken.Communications biology · 2020Article
- Confluence of Cellular Degradation Pathways During Interdigital Tissue Remodeling in Embryonic Tetrapods.Frontiers in cell and developmental biology · 2020Review
- Otic Neurogenesis Is Regulated by TGFβ in a Senescence-Independent Manner.Frontiers in cellular neuroscience · 2020Article
- Cell senescence, apoptosis and DNA damage cooperate in the remodeling processes accounting for heart morphogenesis.Journal of anatomy · 2019Article
- UHRF genes regulate programmed interdigital tissue regression and chondrogenesis in the embryonic limb.Cell death & disease · 2019Article
- Two Proximally Close Priority Candidate Genes for diplopodia-1, an Autosomal Inherited Craniofacial-Limb Syndrome in the Chicken: MRE11 and GPR83.The Journal of heredity · 2019Article
- Tolerance to paternal genotoxic damage promotes survival during embryo development in zebrafish (Biology open · 2018Article
- Interdigital tissue remodelling in the embryonic limb involves dynamic regulation of the miRNA profiles.Journal of anatomy · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA damage independent of caspase activation accompanies programmed cell death in different vertebrate embryonic organs. We analyzed the significance of DNA damage during the regression of the interdigital tissue, which sculpts the digits in the embryonic limb. Interdigit remodeling involves oxidative stress, massive apoptosis and cell senescence. Phosphorylation of H2AX mediated by ATM precedes caspase dependent apoptosis and cell senescence during interdigit regression. The association of γH2AX with other downstream DNA repair factors, including MDC1, Rad50 and 53BP1 suggests a defensive response of cells against DNA damage. The relative distribution of cells γH2AX-only positive, TUNEL-only positive, and cells double positive for both markers is consistent with a sequence of degenerative events starting by damage of the DNA. In support of this interpretation, the relative number of γH2AX-only cells increases after caspase inhibition while the relative number of TUNEL-only cells increases after inhibition of ATM. Furthermore, cultured interdigits survived and maintained intense chondrogenic potential, even at advanced stages of degeneration, discarding a previous commitment to die. Our findings support a new biological paradigm considering embryonic cell death secondary to genotoxic stimuli, challenging the idea that considers physiological cell death a cell suicide regulated by an internal death clock that pre-programmes degeneration.
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