Evidence map›Paper›PMID 27750174›Full record

ArticleProtist2016

The Large Subunit rDNA Sequence of Plasmodiophora brassicae Does not Contain Intra-species Polymorphism.

Arne Schwelm, Cédric Berney, Christina Dixelius, David Bass, Sigrid Neuhauser

Abstract read
In one paragraph

Article in Protist, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

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  8. Molecular Pathotyping ofPathogens (Basel, Switzerland) · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Arne SchwelmSwedish University of Agricultural Sciences, Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, P.O. Box 7080, SE-75007 Uppsala, Sweden; University of Innsbruck, Institute of Microbiology, Technikerstraße 25, 6020 Innsbruck, Austria.
Cédric BerneyDivision of Genomics and Microbial Diversity, Dept of Life Sciences, Natural History Museum London, Cromwell Road, SW7 5BD, UK; Groupe Evolution des Protistes et Ecosystèmes Pélagiques, UMR 7144, CNRS & Université Pierre et Marie Curie, Station Biologique de Roscoff, Place Georges Teissier, 29680 Roscoff, France.
Christina DixeliusSwedish University of Agricultural Sciences, Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, P.O. Box 7080, SE-75007 Uppsala, Sweden.
David BassDivision of Genomics and Microbial Diversity, Dept of Life Sciences, Natural History Museum London, Cromwell Road, SW7 5BD, UK; Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, DT4 8UB, UK.
Sigrid NeuhauserUniversity of Innsbruck, Institute of Microbiology, Technikerstraße 25, 6020 Innsbruck, Austria; Division of Genomics and Microbial Diversity, Dept of Life Sciences, Natural History Museum London, Cromwell Road, SW7 5BD, UK. Electronic address: sigrid.neuhauser@uibk.ac.at.

Funding

Austrian Science Fund FWF J 3175Austrian Science Fund FWF Y 801
6 · The paper itself

Abstract

Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could allow to distinguish pathotypes without the need of time-consuming bioassays. However, isolates of P. brassicae from around the world analysed in this study do not show polymorphism in their LSU rDNA sequences. The previously described polymorphism most likely derived from soil inhabiting Cercozoa more specifically Neoheteromita-like glissomonads. Here we correct the LSU rDNA sequence of P. brassicae. By using FISH we demonstrate that our newly generated sequence belongs to the causal agent of clubroot disease.

Indexed as

Polymorphism, GeneticBrassicaDNA, ProtozoanDNA, RibosomalIn Situ Hybridization, FluorescencePlant DiseasesPlasmodiophoridaDNA, ProtozoanDNA, RibosomalClubrootFISH (fluorescence in-situ hybridisation)LSU rDNApathotypingphytomyxeaplasmodiophorids.

Identifiers

PMID27750174
PMCPMC5221739

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.