ArticleMolecular medicine reports2016
Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference.
Article in Molecular medicine reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 4 citations in OpenAlex.
- Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP.Transgenic research · 2024Article
- Multi-Omics Reveals Different Strategies in the Immune and Metabolic Systems of High-Yielding Strains of Laying Hens.Frontiers in genetics · 2022Article
- Gene expression pattern analysis using dual-color RT-MLPA and integrative genome-wide association studies of eQTL for tuberculosis suscepitibility.Respiratory research · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major cell types expressing Golli in the immune system are the T‑lineage cells. The aim of the current study was to investigate the changes of gene expression in T lymphocytes subsequent to downregulation of the Golli‑myelin basic protein (MBP) gene. RNA interference technology was used to suppress the expression of Golli‑MBP in Jurkat cells and DNA microarray techniques were applied to investigate the alterations of gene expression profiles. The results indicated that there were 387 differentially expressed genes. In the Golli‑MBP knockdown Jurkat cells, the expression of 108 genes was enhanced, 279 genes were suppressed. Gene ontology analysis identified differentially expressed genes involved in several biological progresses, including cell adhesion and immune responses. Pathway analysis demonstrated that the majority of the differentially expressed genes (23.3%) were involved in cytokine‑cytokine receptor interaction. Subsequent to Golli‑MBP knockdown, the mechanisms that changed the biological characteristics of Jurkat cells were complex, involving numerous types of functional proteins, and metabolic and signaling pathways. However, further experiments are required to confirm these results.
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