Evidence map›Paper›PMID 27748416›Full record

ArticleScientific reports2016

MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection.

Qin-Qin He, Liu-Lin Xiong, Fei Liu, Xiang He, Guo-Ying Feng, Fei-Fei Shang, Qing-Jie Xia, You-Cui Wang, De-Lu Qiu, Chao-Zhi Luo and 2 more

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In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Qin-Qin HeInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Liu-Lin XiongInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Fei LiuInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Xiang HeInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Guo-Ying FengDepartment of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Fei-Fei ShangInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Qing-Jie XiaInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
You-Cui WangInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
De-Lu QiuInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Chao-Zhi LuoInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
Jia LiuInstitute of Neuroscience, Animal Zoology Department, Kunming medical University, Kunming 650500, China.
Ting-Hua WangInstitute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neuroregeneration and apoptosis are two important pathophysiologic changes after spinal cord injury (SCI), but their underlying mechanisms remain unclear. MicroRNAs (miRNAs) play a crucial role in the regulation of neuroregeneration and neuronal apoptosis, research areas that have been greatly expanded in recent years. Here, using miRNA arrays to profile miRNA transcriptomes, we demonstrated that miR-127-3p was significantly down-regulated after spinal cord transection (SCT). Then, bioinformatics analyses and experimental detection showed that miR-127-3p exhibited specific effects on the regulation of neurite outgrowth and the induction of neuronal apoptosis by regulating the expression of the mitochondrial membrane protein mitoNEET. Moreover, knockdown of MitoNEET leaded to neuronal loss and apoptosis in primary cultured spinal neurons. This study therefore revealed that miR-127-3p, which targets mitoNEET, plays a vital role in regulating neurite outgrowth and neuronal apoptosis after SCT. Thus, modificatioin of the mitoNEET expression, such as mitoNEET activition may provide a new strategy for the treatment of SCI in preclinical trials.

Indexed as

ApoptosisNerve RegenerationAnimalsBlood-Brain BarrierComputational BiologyDown-RegulationEvoked Potentials, SomatosensoryFemaleGAP-43 ProteinMicroRNAsMitochondrial ProteinsNeuritesNeuronal OutgrowthNeuronsRatsRats, Sprague-DawleyGAP-43 ProteinMicroRNAsMirn127 microRNA, ratmitochondrial protein 17 kDa, ratMitochondrial ProteinsSolute Carrier Family 12, Member 4

Identifiers

PMID27748416
PMCPMC5066253

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.