Evidence map›Paper›PMID 27748364›Full record

ArticleScientific reports2016

Selective Capture of Histidine-tagged Proteins from Cell Lysates Using TEM grids Modified with NTA-Graphene Oxide.

Christopher J Benjamin, Kyle J Wright, Scott C Bolton, Seok-Hee Hyun, Kyle Krynski, Mahima Grover, Guimei Yu, Fei Guo, Tamara L Kinzer-Ursem, Wen Jiang and 1 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 38 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Developing Graphene Grids for Cryoelectron Microscopy.Frontiers in molecular biosciences · 2022
    Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  16. Structural Biology in the Multi-Omics Era.Journal of chemical information and modeling · 2020
    Article
  17. Review
  18. Review
  19. PROTEINS, INTERFACES, AND CRYO-EM GRIDS.Current opinion in colloid & interface science · 2018
    Article
  20. Cryo-EM: beyond the microscope.Current opinion in structural biology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Christopher J BenjaminDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Kyle J WrightDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Scott C BoltonDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Seok-Hee HyunDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Kyle KrynskiDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Mahima GroverDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Guimei YuDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Fei GuoDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Tamara L Kinzer-UrsemWeldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Wen JiangDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
David H ThompsonDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Purdue University West Lafayette · USCenter for Cancer Research · US

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI John Tesmer · 1985 to 2026
$43.4M
Protein Tagging for High Resolution Structural Analysis of Synaptic Protein Complexes Using Clickable CryoEM GridsR21NS095218 · NINDS · PURDUE UNIVERSITY · PI KINZER-URSEM, TAMARA L · 2016 to 2017
$404k
NCI NIH HHS P30 CA023168NINDS NIH HHS R21 NS095218
6 · The paper itself

Abstract

We report the fabrication of transmission electron microscopy (TEM) grids bearing graphene oxide (GO) sheets that have been modified with N

Indexed as

4-Aminobenzoic AcidBacteriophage T7Chaperonin 60Cryoelectron MicroscopyEscherichia coliEscherichia coli ProteinsGraphiteHistidineLysineMembranes, ArtificialMicroscopy, Electron, TransmissionOligopeptidesOxidesProtein BindingRecombinant Fusion Proteins2-N,N-bis(carboxymethyl)lysine4-Aminobenzoic AcidChaperonin 60Escherichia coli ProteinsGraphiteHis-His-His-His-His-HisHistidineLysineMembranes, ArtificialOligopeptidesOxidesRecombinant Fusion Proteins

Identifiers

PMID27748364
PMCPMC5066248
OpenAlexW2534419025

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.