ArticleScientific reports2016
Nanoimaging granule dynamics and subcellular structures in activated mast cells using soft X-ray tomography.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography.Journal of structural biology: X · 2024Article
- Soft X-ray tomography analysis of mitochondria dynamics in Saccharomyces cerevisiae.Biology direct · 2024Article
- Oxidative Stress and Mitochondria Are Involved in Anaphylaxis and Mast Cell Degranulation: A Systematic Review.Antioxidants (Basel, Switzerland) · 2024Review
- Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications.International journal of molecular sciences · 2024Article
- Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro.Veterinary research · 2023Article
- Lipid Droplets, the Central Hub Integrating Cell Metabolism and the Immune System.Frontiers in physiology · 2021Review
- The cellular landscape by cryo soft X-ray tomography.Biophysical reviews · 2019Review
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- Ultraspecific live imaging of the dynamics of zebrafish neutrophil granules by a histopermeable fluorogenic benzochalcone probe.Chemical science · 2019Article
- Remodeling of secretory lysosomes during education tunes functional potential in NK cells.Nature communications · 2019Article
- Across the spectrum: integrating multidimensional metal analytics for in situ metallomic imaging.Metallomics : integrated biometal science · 2019Review
- Baculovirus Utilizes Cholesterol Transporter NIEMANN-Pick C1 for Host Cell Entry.Frontiers in microbiology · 2019Article
- PSF correction in soft X-ray tomography.Journal of structural biology · 2018Article
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria.Plant physiology · 2018Article
- Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells.Emerging topics in life sciences · 2018Article
- 3D membrane segmentation and quantification of intact thick cells using cryo soft X-ray transmission microscopy: A pilot study.PloS one · 2017Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mast cells play an important role in allergic responses. During activation, these cells undergo degranulation, a process by which various kinds of mediators stored in the granules are released. Granule homeostasis in mast cells has mainly been studied by electron microscopy (EM), where the fine structures of subcellular organelles are partially destroyed during sample preparation. Migration and fusion of granules have not been studied in detail in three dimensions (3D) in unmodified samples. Here, we utilized soft X-ray tomography (SXT) coupled with fluorescence microscopy to study the detailed structures of organelles during mast cell activation. We observed granule fission, granule fusion to plasma membranes, and small vesicles budding from granules. We also detected lipid droplets, which became larger and more numerous as mast cells were activated. We observed dramatic morphological changes of mitochondria in activated mast cells and 3D-reconstruction revealed the highly folded cristae inner membrane, features of functionally active mitochondria. We also observed giant vesicles containing granules, mitochondria, and lipid droplets, which we designated as granule-containing vesicles (GCVs) and verified their presence by EM in samples prepared by cryo-substitution, albeit with a less clear morphology. Thus, our studies using SXT provide significant insights into mast cell activation at the organelle level.
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