Evidence map›Paper›PMID 27747294›Full record

ArticleACS sensors2016

High-Throughput Electrochemical Microfluidic Immunoarray for Multiplexed Detection of Cancer Biomarker Proteins.

Chi K Tang, Abhay Vaze, Min Shen, James F Rusling

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 116 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Review
  16. Nano-engineered tools in the diagnosis, therapeutics, prevention, and mitigation of SARS-CoV-2.Journal of controlled release : official journal of the Controlled Release Society · 2021
    Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Chi K TangDepartment of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States.
Abhay VazeDepartment of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States.
Min ShenDepartment of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States.
James F RuslingDepartment of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States; Institute of Materials Science, University of Connecticut, Storrs, Connecticut 06269, United States; Department of Surgery and Neag Cancer Center, University of Connecticut Health Center, Farmington, Connecticut 06032, United States; School of Chemistry, National University of Ireland at Galway, Galway, Ireland.
University of Connecticut · USOllscoil na Gaillimhe – University of Galway · IE

Funding

Protein Biomarker Arrays for Personalized Treatment of Prostate CancerR01EB016707 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2014 to 2017
$1.4M
Protein Biosensor Arrays Based on NanomaterialsR01EB014586 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2011 to 2014
$1.4M
NIBIB NIH HHS R01 EB014586NIBIB NIH HHS R01 EB016707
6 · The paper itself

Abstract

Microchip-based microfluidic electrochemical arrays hold great promise for fast, high-throughput multiplexed detection of cancer biomarker proteins at low cost per assay using relatively simple instrumentation. Here we describe an inexpensive high-throughput electrochemical array featuring 32 individually addressable microelectrodes that is further multiplexed with an 8-port manifold to provide 256 sensors. The gold electrode arrays were fabricated by wet-etching commercial gold compact discs (CD-R) followed by patterned insulation. A print-and-peel method was used to create sub-microliter hydrophobic wells surrounding each sensor to eliminate cross contamination during immobilization of capture antibodies. High-throughput analyses were realized using eight 32-sensor immunoarrays connected to the miniaturized 8-port manifold, allowing 256 measurements in <1 h. This system was used to determine prostate cancer biomarker proteins prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), interleukin-6 (IL-6), and platelet factor-4 (PF-4) in serum. Clinically relevant detection limits (0.05 to 2 pg mL

Indexed as

cancer biomarkers proteinselectrochemical arrayhigh-throughputimmunosensorssensor fabrication

Identifiers

PMID27747294
PMCPMC5058420
OpenAlexW2483709934

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.