ArticleApplied physics letters2016
Unidirectional signal propagation in primary neurons micropatterned at a single-cell resolution.
Article in Applied physics letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A Dual-Function Micropatterned Gold Platform for Cell Patterning and Optical Imaging Applications.Sensors and actuators. B, Chemical · 2025Article
- Nonreciprocal surface plasmonic neural network for decoupled bidirectional analogue computing.Nature communications · 2025Article
- Open-End Control of Neurite Outgrowth Lengths with Steep Bending Confinement Microchannel Patterns for Miswiring-Free Neuronal Network Formation.Micromachines · 2024Article
- Network Bursts in 3D Neuron Clusters Cultured on Microcontact-Printed Substrates.Micromachines · 2023Article
- Biological neurons act as generalization filters in reservoir computing.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- An Overview of In Vitro Biological Neural Networks for Robot Intelligence.Cyborg and bionic systems (Washington, D.C.) · 2023Review
- Neurons-on-a-Chip:Molecules and cells · 2022Review
- Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks.Frontiers in neuroscience · 2022Article
- Stepwise neuronal network pattern formation in agarose gel during cultivation using non-destructive microneedle photothermal microfabrication.Scientific reports · 2021Article
- Microdevice for directional axodendritic connectivity between micro 3D neuronal cultures.Microsystems & nanoengineering · 2021Article
- Thermoplasmonic neural chip platform for in situ manipulation of neuronal connections in vitro.Nature communications · 2020Article
- Surface engineering within a microchannel for hydrodynamic and self-assembled cell patterning.Biomicrofluidics · 2020Article
- Single-cell patterning technology for biological applications.Biomicrofluidics · 2019Review
- Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement.Micromachines · 2019Article
- Impact of modular organization on dynamical richness in cortical networks.Science advances · 2018Article
- Dependence and Homeostasis of Membrane Impedance on Cell Morphology in Cultured Hippocampal Neurons.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure and connectivity of cultured neuronal networks can be controlled by using micropatterned surfaces. Here, we demonstrate that the direction of signal propagation can be precisely controlled at a single-cell resolution by growing primary neurons on micropatterns. To achieve this, we first examined the process by which axons develop and how synapses form in micropatterned primary neurons using immunocytochemistry. By aligning asymmetric micropatterns with a marginal gap, it was possible to pattern primary neurons with a directed polarization axis at the single-cell level. We then examined how synapses develop on micropatterned hippocampal neurons. Three types of micropatterns with different numbers of short paths for dendrite growth were compared. A normal development in synapse density was observed when micropatterns with three or more short paths were used. Finally, we performed double patch clamp recordings on micropatterned neurons to confirm that these synapses are indeed functional, and that the neuronal signal is transmitted unidirectionally in the intended orientation. This work provides a practical guideline for patterning single neurons to design functional neuronal networks
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