ArticleDevelopmental cell2016
A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization.
Article in Developmental cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
176 citing papers in PubMed, 363 citations in OpenAlex.
- Optogenetic BMP4 models symmetry breaking and primitive streak formation in hPSCs.bioRxiv : the preprint server for biology · 2026Article
- Flexible high-resolution ECM micropatterning.Nature protocols · 2026Review
- Biochemical principles of SMAD signaling across the animal kingdom.The Biochemical journal · 2026Review
- AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.Developmental cell · 2026Article
- The notochord: development, disease and stem cell-based modelling.Development (Cambridge, England) · 2026Review
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- Self-organized anteroposterior regionalization of early midbrain and hindbrain/spinal cords using micropatterned human pluripotent stem cells.SLAS technology · 2026Article
- Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit.bioRxiv : the preprint server for biology · 2026Article
- TDP43 and hnRNP K Regulate Alternative Splicing of DNAJC5.Cell biology international · 2026Article
- Optogenetic WNT signaling drives germ layer self-organization in a human gastruloid model.bioRxiv : the preprint server for biology · 2026Article
- FATE-MAP predicts teratogenicity and human gastrulation failure modes by integrating deep learning and mechanistic modeling.Nature communications · 2026Article
- Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids.Development (Cambridge, England) · 2026Article
- Human stem cell-based embryo models: from self-organization to recapitulating development.Frontiers in cell and developmental biology · 2026Review
- Optogenetic Rescue Reveals Spatiotemporal Rules of Germ-Layer Patterning.bioRxiv : the preprint server for biology · 2025Article
- mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation.Stem cell reports · 2025Article
- Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids.bioRxiv : the preprint server for biology · 2025Article
- Juxtaposition of human pluripotent stem cells with amnion-like cells is sufficient to trigger primitive streak formation.bioRxiv : the preprint server for biology · 2025Article
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
116 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The earliest aspects of human embryogenesis remain mysterious. To model patterning events in the human embryo, we used colonies of human embryonic stem cells (hESCs) grown on micropatterned substrate and differentiated with BMP4. These gastruloids recapitulate the embryonic arrangement of the mammalian germ layers and provide an assay to assess the structural and signaling mechanisms patterning the human gastrula. Structurally, high-density hESCs localize their receptors to transforming growth factor β at their lateral side in the center of the colony while maintaining apical localization of receptors at the edge. This relocalization insulates cells at the center from apically applied ligands while maintaining response to basally presented ones. In addition, BMP4 directly induces the expression of its own inhibitor, NOGGIN, generating a reaction-diffusion mechanism that underlies patterning. We develop a quantitative model that integrates edge sensing and inhibitors to predict human fate positioning in gastruloids and, potentially, the human embryo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.