ArticleSteroids2016
Validation of highly sensitive simultaneous targeted and untargeted analysis of keto-steroids by Girard P derivatization and stable isotope dilution-liquid chromatography-high resolution mass spectrometry.
Article in Steroids, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Ovarian-Specific Cyp17A1 Overexpression in Female Mice: A Novel Model of Endogenous Testosterone Excess.Endocrinology · 2025Article
- Unveiling the global landscape of anabolic steroid residues in beef via integrated targeted quantification and high-resolution suspect screening.Current research in food science · 2025Article
- Characterization of spironolactone and metabolites derivatized using Girard's reagent P using mass spectrometry and ion mobility spectrometry.Rapid communications in mass spectrometry : RCM · 2024Article
- High sensitivity LC-MS methods for quantitation of hydroxy- and keto-androgens.Methods in enzymology · 2023Article
- Maternal androgens and autism spectrum disorder in the MARBLES prospective cohort study.Research in autism spectrum disorders · 2022Article
- Conversion of Classical and 11-Oxygenated Androgens by Insulin-Induced AKR1C3 in a Model of Human PCOS Adipocytes.Endocrinology · 2022Article
- Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS.Molecules (Basel, Switzerland) · 2021Review
- Parallel, High-Quality Proteomic and Targeted Metabolomic Quantification Using Laser Capture Microdissected Tissues.Analytical chemistry · 2021Article
- Meconium androgens are correlated with ASD-related phenotypic traits in early childhood in a familial enriched risk cohort.Molecular autism · 2020Article
- Association of serum androgens and coronary artery calcium scores in women.Fertility and sterility · 2019Article
- Pd-catalyzed asymmetric allylic substitution cascade using α-(pyridin-1-yl)-acetamides formedChemical science · 2019Article
- Mice exposed to bisphenol A exhibit depressive-like behavior with neurotransmitter and neuroactive steroid dysfunction.Hormones and behavior · 2018Article
- Relationship of SULT1A1 copy number variation with estrogen metabolism and human health.The Journal of steroid biochemistry and molecular biology · 2017Article
- Susceptibility to traumatic stress sensitizes the dopaminergic response to cocaine and increases motivation for cocaine.Neuropharmacology · 2017Article
- Differences in testosterone and its precursors by sex of the offspring in meconium.The Journal of steroid biochemistry and molecular biology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
A multiplexed quantitative method for the analysis of three major unconjugated steroids in human serum by stable isotope dilution liquid chromatography-high resolution mass spectrometry (LC-HRMS) was developed and validated on a Q Exactive Plus hybrid quadrupole/Orbitrap mass spectrometer. This quantification utilized isotope dilution and Girard P derivatization on the keto-groups of testosterone (T), androstenedione (AD) and dehydroepiandrosterone (DHEA) to improve ionization efficiency using electrospray ionization. Major isomeric compounds to T and DHEA; the inactive epimer of testosterone (epiT), and the metabolite of AD, 5α-androstanedione (5α-AD) were completely resolved on a biphenyl column within an 18min method. Inter- and intra-day method validation using LC-HRMS with qualifying product ions was performed and acceptable analytical performance was achieved. The method was further validated by comparing steroid levels from 100μL of serum from young vs older subjects. Since this approach provides high-dimensional HRMS data, untargeted analysis by age group was performed. DHEA and T were detected among the top analytes most significantly different across the two groups after untargeted LC-HRMS analysis, as well as a number of other still unknown metabolites, indicating the potential for combined targeted/untargeted analysis in steroid analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.