ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2016
Compared antioxidant activity among corticosteroids on cultured retinal pigment epithelial cells.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 8 citations in OpenAlex.
- Epigenetic alterations associated with dexamethasone sodium phosphate through DNMT and TET in RPE cells.Molecular vision · 2021Article
- Internal limiting membrane peeling with different dyes in the surgery of idiopathic macular hole: a systematic review of literature and network Meta-analysis.International journal of ophthalmology · 2019Article
- Glucocorticoid receptor signaling in the eye.Steroids · 2018Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study is to determine if dexamethasone, prednisolone and triamcinolone acetonide (TA), three anti-inflammatory drugs commonly used for ocular treatments, could affect the oxidative status of cultured human cells of the retinal pigment epithelium (RPE) and protect them against oxidative injury.
methodsARPE-19 cells were used as an in vitro model of RPE. Glutathione (GSH) levels were assessed to evaluate the effects of dexamethasone, prednisolone and triamcinolone on cellular antioxidant status. Oxidative stress was induced in ARPE-19 cells by treatment with the oxidizing agent menadione, and the effects of dexamethasone, prednisolone and triamcinolone were evaluated. Release of lactate dehydrogenase (LDH) in the culture medium was used to measure cytotoxicity.
resultsIncubation with triamcinolone or prednisolone was not able to revert menadione-induced cytotoxicity and GSH depletion; furthermore, it significantly decreased GSH levels in ARPE-19 cells (nmol of GSH/mg cellular protein: 99.7 ± 0.1 in untreated controls vs. 52.6 ± 5.2 with triamcinolone vs. 77.6 ± 5.2 with prednisolone; p < 0.001). Treatment with dexamethasone protected ARPE-19 cells from cytotoxicity and oxidative damage: lactate dehydrogenase release and GSH depletion were significantly decreased after incubation with this compound (LDHout/LDHtot: 0.221 ± 0.038 with menadione vs. 0.041 ± 0.007 with menadione + dexamethasone; p < 0.001; nmol of GSH/mg cellular protein: 5.7 ± 4.2 with menadione vs. 53.2 ± 6.1 with menadione + dexamethasone, respectively; p < 0.001) and did not induce GSH depletion (nmol of GSH/mg cellular protein: 99.7 ± 0.1 vs. 86.5 ± 8.1 nmol/min/mg prot with dexamethasone; p > 0.05).
conclusionsDexamethasone, besides suppressing intraocular inflammation, may protect human RPE cells from oxidative stress and decrease the oxidation rate of GSH. Triamcinolone and prednisolone, inducing GSH depletion, may contribute to reduce antioxidant capacity of ARPE-19 cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.