ReviewCold Spring Harbor perspectives in biology2016
Cell-Free Synthetic Biology: Engineering Beyond the Cell.
Review in Cold Spring Harbor perspectives in biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed.
- Evolution of Whole-Cell Biosensor Detection Technology for PAHs and Their Halogenated Derivatives Driven by Performance Requirements.Biosensors · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Cell-Free Genetic Circuits: Extract and Template Preparation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Rethinking governance of synthetic cells.Frontiers in bioengineering and biotechnology · 2026Article
- FASTIA: A rapid and accessible platform for protein variant interaction analysis demonstrated with a single-domain antibody.Protein science : a publication of the Protein Society · 2025Article
- Functionality and translation fidelity characterization of mRNA vaccines using platform based mass spectrometry detection.NPJ vaccines · 2025Article
- Cell-Free Systems and Their Importance in the Study of Membrane Proteins.The Journal of membrane biology · 2025Review
- ATP Regeneration from Pyruvate in the PURE System.ACS synthetic biology · 2025Article
- Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.ACS synthetic biology · 2025Article
- Detection of environmental pollutants using transcription factor-based whole-cell biosensors.Biotechnology for the environment · 2025Review
- Exploring cost reduction strategies for serum free media development.NPJ science of food · 2024Review
- Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system.Odontology · 2024Article
- Applications of cell free protein synthesis in protein design.Protein science : a publication of the Protein Society · 2024Review
- Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems.Chemical reviews · 2024Review
- Functional analysis of the AUG initiator codon context reveals novel conserved sequences that disfavor mRNA translation in eukaryotes.Nucleic acids research · 2024Article
- A Cost-EffectiveBioengineering (Basel, Switzerland) · 2024Article
- Toehold switch plus signal amplification enables rapid detection.Biotechnology journal · 2023Article
- Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- A Role for Bottom-Up Synthetic Cells in the Internet of Bio-Nano Things?Molecules (Basel, Switzerland) · 2023Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Cell-free protein synthesis (CFPS) technologies have enabled inexpensive and rapid recombinant protein expression. Numerous highly active CFPS platforms are now available and have recently been used for synthetic biology applications. In this review, we focus on the ability of CFPS to expand our understanding of biological systems and its applications in the synthetic biology field. First, we outline a variety of CFPS platforms that provide alternative and complementary methods for expressing proteins from different organisms, compared with in vivo approaches. Next, we review the types of proteins, protein complexes, and protein modifications that have been achieved using CFPS systems. Finally, we introduce recent work on genetic networks in cell-free systems and the use of cell-free systems for rapid prototyping of in vivo networks. Given the flexibility of cell-free systems, CFPS holds promise to be a powerful tool for synthetic biology as well as a protein production technology in years to come.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.