ArticleAnalytical biochemistry2017
Notes and tips for improving quality of lipid-protein overlay assays.
Article in Analytical biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A lipid-binding protein in black-legged tick saliva selectively recognizesbioRxiv : the preprint server for biology · 2026Article
- Article
- Regulation of STING activation by phosphoinositide and cholesterol.bioRxiv : the preprint server for biology · 2025Article
- Characterization of the malaria parasiteMicrobiology spectrum · 2025Article
- LPS binding caspase activation and recruitment domains (CARDs) are bipartite lipid binding modules.Science advances · 2025Article
- The Influence of Phosphoinositide Lipids in the Molecular Biology of Membrane Proteins: Recent Insights from Simulations.Journal of molecular biology · 2025Review
- Roles of non-specific lipid transfer proteins in plant defense: structural and functional perspectives.Frontiers in fungal biology · 2025Review
- LPS binding caspase activation and recruitment domains (CARDs) are bipartite lipid binding modules.bioRxiv : the preprint server for biology · 2024Article
- CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling.Nature communications · 2023Article
- Review
- YPIBP: A repository for phosphoinositide-binding proteins in yeast.Computational and structural biotechnology journal · 2021Article
- Molecular characterization of a fungal gasdermin-like protein.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Lipid Interactions of a Ciliary Membrane TRP Channel: Simulation and Structural Studies of Polycystin-2.Structure (London, England : 1993) · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis.EMBO reports · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
To reduce costs of lipid-binding assays, allow for multiple lipids to be screened for protein binding simultaneously, and to make lipid binding more user friendly, lipids have been dotted onto membranes to investigate lipid-protein interactions. These assays are similar to a western blot where the membrane is blocked, incubated with a protein of interest and detected using antibodies. Although the assay is inexpensive and straightforward, problems with promiscuous or poor binding, as well as insufficient blocking occur frequently. In this technical note, we share several specific improvements to ensure lipid-protein overlay assays are of high quality and contain proper controls.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.