Evidence map›Paper›PMID 27741498›Full record

ArticleBiomaterials2017

In vitro 3D corneal tissue model with epithelium, stroma, and innervation.

Siran Wang, Chiara E Ghezzi, Rachel Gomes, Rachel E Pollard, James L Funderburgh, David L Kaplan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Progress in the application of 3D printing technology in ophthalmology.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023
    Review
  12. Article
  13. Article
  14. Article
  15. Selection of natural biomaterials for micro-tissue and organ-on-chip models.Journal of biomedical materials research. Part A · 2022
    Review
  16. Fiber-Based Biopolymer Processing as a Route toward Sustainability.Advanced materials (Deerfield Beach, Fla.) · 2022
    Review
  17. Tissue Models forFrontiers in cellular and infection microbiology · 2022
    Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Siran WangNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215021, People's Republic of China; Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
Chiara E GhezziDepartment of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
Rachel GomesDepartment of Biomedical Engineering, Tufts University, Medford, MA 02155, USA; New England Eye Center, Tufts Medical Center, Boston, MA, USA; Federal University of São Paulo, School of Medicine, São Paulo, Brazil.
Rachel E PollardDepartment of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
James L FunderburghDepartment of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
David L KaplanNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215021, People's Republic of China; Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Electronic address: david.kaplan@tufts.edu.

Funding

Stem Cells for Corneal EngineeringR01EY016415 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI DU, YIQIN · 2006 to 2018
$4.5M
Tissue Engineering Cornea ReplacementsR01EY020856 · NEI · TUFTS UNIVERSITY MEDFORD · PI KAPLAN, DAVID L. · 2010 to 2016
$2.7M
NEI NIH HHS R01 EY016415NEI NIH HHS R01 EY020856
6 · The paper itself

Abstract

The interactions between corneal nerve, epithelium, and stroma are essential for maintaining a healthy cornea. Thus, corneal tissue models that more fully mimic the anatomy, mechanical properties and cellular components of corneal tissue would provide useful systems to study cellular interactions, corneal diseases and provide options for improved drug screening. Here a corneal tissue model was constructed to include the stroma, epithelium, and innervation. Thin silk protein film stacks served as the scaffolding to support the corneal epithelial and stromal layers, while a surrounding silk porous sponge supported neuronal growth. The neurons innervated the stromal and epithelial layers and improved function and viability of the tissues. An air-liquid interface environment of the corneal tissue was also mimicked in vitro, resulting in a positive impact on epithelial maturity. The inclusion of three cell types in co-culture at an air-liquid interface provides an important advance for the field of in vitro corneal tissue engineering, to permit improvements in the study of innervation and corneal tissue development, corneal disease, and tissue responses to environmental factors.

Indexed as

Printing, Three-DimensionalBioartificial OrgansCells, CulturedCoculture TechniquesCorneaHumansNeuronsOrgan Culture TechniquesSilkStromal CellsTissue EngineeringTissue ScaffoldsSilkCorneaInnervationSilkStromaTissue model

Identifiers

PMID27741498
PMCPMC5121002

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.