ArticlePloS one2016
A High-Yield Two-Hour Protocol for Extraction of Human Hair Shaft Proteins.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Enhanced Genetically Variant Peptide Profiling from Human Hair Using Multiple Enzymes.ACS omega · 2026Article
- Pepsin Digestion for Proteomic Studies of the Human Hair Shaft.Rapid communications in mass spectrometry : RCM · 2026Article
- Extraction of keratin particles as intact protein sequences from chicken feathers and their characterization.Biomaterials and biosystems · 2026Article
- Advantages of Incomplete Digestion in Human Hair Shaft Proteomics, a Focus on Cuticular Keratins.Rapid communications in mass spectrometry : RCM · 2025Article
- Improved Sample Preparation Method for Protein and Peptide Identification from Human Hair.Journal of proteome research · 2024Article
- Human Hair Keratin Composite Scaffold: Characterisation and Biocompatibility Study on NIH 3T3 Fibroblast Cells.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Sensitive Method for the Confident Identification of Genetically Variant Peptides in Human Hair Keratin.Journal of forensic sciences · 2020Article
- Potential use of human hair shaft keratin peptide signatures to distinguish gender and ethnicity.PeerJ · 2020Article
- Identification of Hybrid Insulin Peptides (HIPs) in Mouse and Human Islets by Mass Spectrometry.Journal of proteome research · 2019Article
- Development of high-performance two-dimensional gel electrophoresis for human hair shaft proteome.PloS one · 2019Article
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Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteome analysis of the human hair remains challenging due to the poor solubility of hair proteins and the difficulty in their extraction. In the present study, we have developed a rapid extraction protocol for hair shaft protein using alkaline-based buffer. The new protocol accelerated the procedure by reducing the extraction time from at least a day to less than two hours and showed a protein recovery of 47.3 ± 3.72%. Further analyses of the extracted protein sample through sodium dodecyl sulfate polyacrylamide gel electrophoresis and Quadrupole-time-of-flight mass spectrometry analysis unveiled a total of 60 proteins, including 25 that were not previously reported. Identification of these proteins is anticipated to be crucial in helping to understand the molecular basis of hair for potential applications in the future.
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Registered trials
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