ArticlePloS one2016
Identification of a Desaturase Involved in Mycolic Acid Biosynthesis in Mycobacterium smegmatis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Comparative genomics and molecular insights into smooth and rough clinical isolates ofMicrobial genomics · 2026Article
- Getting a grip on StAR-related lipid-transfer (START) domain proteins in bacteria.FEMS microbiology reviews · 2026Review
- The Biosynthetic Pathway of Mycolic Acids: Dual-Function Targets for Tuberculosis Therapeutics and Green Steroid Drugs Biomanufacturing.Pharmaceutics · 2025Review
- Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria.Nucleic acids research · 2025Article
- Mycobacterium tuberculosis curli pili (MTP) and heparin-binding hemagglutinin adhesin (HBHA) facilitate regulation of central carbon metabolism, enhancement of ATP synthesis and cell wall biosynthesis.Archives of microbiology · 2025Article
- Identification of novel single nucleotide variants in the drug resistance mechanism of Mycobacterium tuberculosis isolates by whole-genome analysis.BMC genomics · 2024Article
- Transcriptomic responses to antibiotic exposure inAntimicrobial agents and chemotherapy · 2024Article
- Comparative proteomic investigation unravels the pathobiology of Mycobacterium fortuitum biofilm.Applied microbiology and biotechnology · 2023Article
- Comprehensive essentiality analysis of themBio · 2023Article
- Ion mobility mass spectrometry for the study of mycobacterial mycolic acids.Scientific reports · 2023Article
- Article
- The mycobacterial desaturase DesA2 is associated with mycolic acid biosynthesis.Scientific reports · 2022Article
- MadR mediates acyl CoA-dependent regulation of mycolic acid desaturation in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A reversed phase ultra-high-performance liquid chromatography-data independent mass spectrometry method for the rapid identification of mycobacterial lipids.Journal of chromatography. A · 2022Article
- Article
- Single START-domain protein Mtsp17 is involved in transcriptional regulation in Mycobacterium smegmatis.PloS one · 2021Article
- The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis.BMC microbiology · 2020Article
- 3-Antimicrobial agents and chemotherapy · 2019Article
- Path-seq identifies an essential mycolate remodeling program for mycobacterial host adaptation.Molecular systems biology · 2019Article
- Construction and Use of TransposonFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Mycolic acids are unique long chain fatty acids found in the cell walls of mycobacteria including the tubercle bacillus, Mycobacterium tuberculosis. The introduction of double bonds in mycolic acids remains poorly understood, however, genes encoding two potential aerobic desaturases have been proposed to be involved in this process. Here we show that one of these genes, desA1, is essential for growth of the saprophytic Mycobacterium smegmatis. Depletion of desA1 in a M. smegmatis conditional mutant led to reduction of mycolic acid biosynthesis and loss of viability. The DesA1-depleted cells exhibited two other phenotypes: using 14[C]-labelling, we detected the accumulation of minor mycolic acid-related species that migrated faster in a silver TLC plate. Spiral Time of Flight Mass Spectroscopic analysis suggested the presence of species with sizes corresponding to what were likely monoenoic derivatives of α-mycolic acids. Additionally, conditional depletion led to the presence of free fatty acyl species of lengths ~C26-C48 in the lysing cells. Cell viability could be rescued in the conditional mutant by Mycobacterium tuberculosis desA1, highlighting the potential of desA1 as a new drug target in pathogenic mycobacteria.
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