ArticleScientific reports2016
Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Optimization and Parallelization of Sorting by Interfacial Tension (SIFT) for High-Throughput Metabolic Cell Sorting.Micromachines · 2026Article
- Stepwise isolation of diverse metabolic cell populations using sorting by interfacial tension (SIFT).Lab on a chip · 2025Article
- Deep learning assisted holography microscopy for in-flow enumeration of tumor cells in blood.RSC advances · 2023Article
- Mechanobiology and survival strategies of circulating tumor cells: a process towards the invasive and metastatic phenotype.Frontiers in cell and developmental biology · 2023Review
- Comparison of sample preparation methods for rare cell isolation in microfluidic devices.Canadian journal of chemistry · 2022Article
- Numerical Study of a Centrifugal Platform for the Inertial Separation of Circulating Tumor Cells Using Contraction-Expansion Array Microchannels.Archives of Razi Institute · 2022Article
- Cancer cell mechanobiology: a new frontier for cancer research.Journal of the National Cancer Center · 2022Review
- Microfluidic-Based Technologies for CTC Isolation: A Review of 10 Years of Intense Efforts towards Liquid Biopsy.International journal of molecular sciences · 2022Review
- Effective Drug Concentration and Selectivity Depends on Fraction of Primitive Cells.International journal of molecular sciences · 2021Article
- Inertial Microfluidics Enabling Clinical Research.Micromachines · 2021Review
- Advances in Tumor Sampling and Sequencing in Breast Cancer and their Application in Precision Diagnostics and Therapeutics.Advances in experimental medicine and biology · 2021Article
- Stiffness based enrichment of leukemia cells using microfluidics.APL bioengineering · 2020Article
- Quantification of Protein Secretion from Circulating Tumor Cells in Microfluidic Chambers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis.Analytical chemistry · 2020Article
- Label-free counting of affinity-enriched circulating tumor cells (CTCs) using a thermoplastic micro-Coulter counter (μCC).The Analyst · 2020Article
- Experimental Study on Microfluidic Mixing with Different Zigzag Angles.Micromachines · 2019Article
- Investigating circulating tumor cells and distant metastases in patient-derived orthotopic xenograft models of triple-negative breast cancer.Breast cancer research : BCR · 2019Article
- Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis - towards cell processing of complex biological samples.Scientific reports · 2019Article
- Creating a capture zone in microfluidic flow greatly enhances the throughput and efficiency of cancer detection.Biomaterials · 2019Article
- Single-Cell Analysis of Circulating Tumor Cells: How Far Have We Come in the -Omics Era?Frontiers in genetics · 2019Review
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
Circulating tumor cells (CTCs) have a great potential as indicators of metastatic disease that may help physicians improve cancer prognostication, treatment and patient outcomes. Heterogeneous marker expression as well as the complexity of current antibody-based isolation and analysis systems highlights the need for alternative methods. In this work, we use a microfluidic Vortex device that can selectively isolate potential tumor cells from blood independent of cell surface expression. This system was adapted to interface with three protein-marker-free analysis techniques: (i) an in-flow automated image processing system to enumerate cells released, (ii) cytological analysis using Papanicolaou (Pap) staining and (iii) fluorescence in situ hybridization (FISH) targeting the ALK rearrangement. In-flow counting enables a rapid assessment of the cancer-associated large circulating cells in a sample within minutes to determine whether standard downstream assays such as cytological and cytogenetic analyses that are more time consuming and costly are warranted. Using our platform integrated with these workflows, we analyzed 32 non-small cell lung cancer (NSCLC) and 22 breast cancer patient samples, yielding 60 to 100% of the cancer patients with a cell count over the healthy threshold, depending on the detection method used: respectively 77.8% for automated, 60-100% for cytology, and 80% for immunostaining based enumeration.
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Registered trials
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