ArticleScientific reports2016
Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.GM crops & food · 2026Article
- Validation of a Duplex Digital PCR Assay for the Quantification of the NK603 Maize Event Across Three dPCR Platforms.Foods (Basel, Switzerland) · 2026Article
- Comparison of two digital PCR platforms for quantification of genetically modified soybean events.GM crops & food · 2025Article
- Nanomaterials for bacterial enrichment and detection in healthcare.Nanomedicine (London, England) · 2025Review
- Detection of Soybean GMO Events Using Two Multiplex Droplet Digital PCR Assays.Journal of AOAC International · 2025Article
- Article
- Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR.Foods (Basel, Switzerland) · 2024Article
- In-House Validation of Four Duplex Droplet Digital PCR Assays to Quantify GM Soybean Events.Foods (Basel, Switzerland) · 2024Article
- Development of a Tetraplex Digital PCR Assay for the Detection of Invasive Snake Species in Florida, USA.Ecology and evolution · 2024Article
- Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes.FEMS microbiology reviews · 2024Review
- Spinal muscular atrophy: Molecular mechanism of pathogenesis, diagnosis, therapeutics, and clinical trials in the Indian context.Journal of biosciences · 2024Review
- Fast and Accurate Multiplex Identification and Quantification of Seven Genetically Modified Soybean Lines Using Six-Color Digital PCR.Foods (Basel, Switzerland) · 2023Article
- Digital PCR cluster predictor: a universal R-package and shiny app for the automated analysis of multiplex digital PCR data.Bioinformatics (Oxford, England) · 2023Article
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Droplet Encoding-Pairing Enabled Multiplexed Digital Loop-Mediated Isothermal Amplification for Simultaneous Quantitative Detection of Multiple Pathogens.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A home-made pipette droplet microfluidics rapid prototyping and training kit for digital PCR, microorganism/cell encapsulation and controlled microgel synthesis.Scientific reports · 2023Article
- Modelling clinical DNA fragmentation in the development of universal PCR-based assays for bisulfite-converted, formalin-fixed and cell-free DNA sample analysis.Scientific reports · 2022Article
- Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks.Clinical microbiology reviews · 2022Review
- Granulocyte colony-stimulating factor protected against brain injury in a rat cerebral hemorrhage model by modulating inflammation.Experimental animals · 2022Article
- Expression of GM content in mass fraction from digital PCR data.Food control · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets.
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Registered trials
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