ArticleScientific reports2016
Selective detection of viable seed-borne Acidovorax citrulli by real-time PCR with propidium monoazide.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Novel and Advanced Diagnostic Approach toward Paracidovorax citrulli Causing Bacterial Fruit Blotch in Watermelon by Direct SYBR Green Real-Time PCR Assay.The plant pathology journal · 2024Article
- Advances in the detection technology of vegetable soil borne fungi and bacteria.Frontiers in microbiology · 2024Review
- An Improved Method for Quantification of ViableMicroorganisms · 2022Article
- The calcium cyanamide and polyethylene blocks the secondary transmission and infection of vegetable leaf diseases.Frontiers in plant science · 2022Article
- Double antibody pairs sandwich-ELISA (DAPS-ELISA) detects Acidovorax citrulli serotypes with broad coverage.PloS one · 2020Article
- Development of Molecular Markers for Detection of Acidovorax citrulli Strains Causing Bacterial Fruit Blotch Disease in Melon.International journal of molecular sciences · 2019Article
- Direct detection from clinical sputum samples to differentiate live and dead Mycobacterium Tuberculosis.Journal of clinical laboratory analysis · 2019Article
- Article
- Induction and Resuscitation of the Viable but Non-culturable (VBNC) State inFrontiers in microbiology · 2019Article
- Involvement ofFrontiers in microbiology · 2018Article
- Detection of Clavibacter michiganensis subsp. michiganensis in viable but nonculturable state from tomato seed using improved qPCR.PloS one · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, use of the DNA-intercalating dye propidium monoazide (PMA) in real-time PCR has been reported as a novel method to detect viable bacteria in different types of samples, such as food, environmental, and microbiological samples. In this study, viable cells of Acidovorax citrulli, the causal agent of bacterial seedling blight and fruit blotch, were selectively detected and differentiated from dead cells by real-time fluorescent polymerase chain reaction amplification after the bacterial solution was treated with the DNA-binding dye PMA. The primers and TaqMan probe were based on the A. citrulli genome (Aave_1909, Gene ID: 4669443) and were highly specific for A. citrulli. The detection threshold of this assay was 10
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