ArticleScientific reports2016
A nuclear-replicating viroid antagonizes infectivity and accumulation of a geminivirus by upregulating methylation-related genes and inducing hypermethylation of viral DNA.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Genome-wide DNA methylation analysis of CBCVd-infected hop plants (Microbiology spectrum · 2025Article
- Characterisation of a Betasatellite Associated With Tomato Yellow Leaf Curl Guangdong Virus and Discovery of an Unusual Modulation of Virus Infection Associated With C4 Protein.Molecular plant pathology · 2025Article
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- PSTVd infection inFrontiers in plant science · 2023Article
- DNA methylation dynamics in response to abiotic and pathogen stress in plants.Plant cell reports · 2022Review
- Article
- Degradome Analysis of Tomato andInternational journal of molecular sciences · 2021Article
- Transcriptome and DNA Methylome Reveal Insights Into Phytoplasma Infection Responses in Mulberry (Frontiers in plant science · 2021Article
- Article
- How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host.RNA biology · 2019Article
- Integrated single-base resolution maps of transcriptome, sRNAome and methylome of Tomato yellow leaf curl virus (TYLCV) in tomato.Scientific reports · 2019Article
- Small RNA-Omics for Plant Virus Identification, Virome Reconstruction, and Antiviral Defense Characterization.Frontiers in microbiology · 2018Review
- Viroid-Derived siRNAs: Minimal RNAs Driving Multiscale Perturbations of Host Regulatory Systems.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation and post-transcriptional gene silencing play critical roles in controlling infection of single-stranded (ss) DNA geminiviruses and ssRNA viroids, respectively, but both pathogens can counteract these host defense mechanisms and promote their infectivity. Moreover, a specific role of DNA methylation in viroid-host interactions is not yet confirmed. Here, using an experimental system where two nuclear-replicating agents, the geminivirus tomato yellow leaf curl Sardinia virus (TYLCSV) and potato spindle tuber viroid (PSTVd), co-infect their common host tomato, we observed that PSTVd severely interferes with TYLCSV infectivity and accumulation, most likely as a consequence of strong activation of host DNA methylation pathways. In fact, PSTVd alone or in co-infection with TYLCSV significantly upregulates the expression of key genes governing DNA methylation in plants. Using methylation-sensitive restriction and bisulfite conversion assays, we further showed that PSTVd infection promotes a strong hypermethylation of TYLCSV DNA, thus supporting a mechanistic link with the antagonism of the viroid on the virus in co-infected tomato plants. These results describe the interaction between two nuclear-replicating pathogens and show that they differentially interfere with DNA methylation pathways.
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