ArticleJournal of neurovirology2017
Pseudo-typed Semliki Forest virus delivers EGFP into neurons.
Article in Journal of neurovirology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Trans-Amplifying RNA Vaccines Against Infectious Diseases: A Comparison with Non-Replicating and Self-Amplifying RNA.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Viral Tools for Neural Circuit Tracing.Neuroscience bulletin · 2022Review
- Magneto is ineffective in controlling electrical properties of cerebellar Purkinje cells.Nature neuroscience · 2020Article
- A Trans-amplifying RNA Vaccine Strategy for Induction of Potent Protective Immunity.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution.National science review · 2019Article
- Anterior cingulate cortex dysfunction underlies social deficits in Shank3 mutant mice.Nature neuroscience · 2019Article
- Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.Neuroscience bulletin · 2019Article
- A Student's Guide to Neural Circuit Tracing.Frontiers in neuroscience · 2019Review
- Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing.Frontiers in neuroanatomy · 2019Article
- MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.Frontiers in cellular neuroscience · 2019Article
- Review
- Ablation of TFR1 in Purkinje Cells Inhibits mGlu1 Trafficking and Impairs Motor Coordination, But Not Autistic-Like Behaviors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semliki Forest virus (SFV), a neurotropic virus, has been used to deliver heterologous genes into cells in vitro and in vivo. In this study, we constructed a reporter SFV4-FL-EGFP and found that it can deliver EGFP into neurons located at the injection site without disseminating throughout the brain. Lacking of the capsid gene of SFV4-FL-EGFP does not block its life cycle, while forming replication-competent virus-like particles (VLPs). These VLPs hold subviral genome by using the packaging sequence (PS) located within the nsP2 gene, and can transfer their genome into cells. In addition, we found that the G protein of vesicular stomatitis virus (VSVG) can package SFV subviral genome, which is consistent with the previous reports. The G protein of rabies virus (RVG) could also package SFV subviral genome. These pseudo-typed SFV can deliver EGFP gene into neurons. Taken together, these findings may be used to construct various SFV-based delivery systems for virological studies, gene therapy, and neural circuit labeling.
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Registered trials
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