ArticleCellular logistics
An improved reversibly dimerizing mutant of the FK506-binding protein FKBP.
Article in Cellular logistics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Functional assignment of Golgi-associated vesicle tethers to specific membrane recycling pathways.bioRxiv : the preprint server for biology · 2026Article
- COPI-dependent intra-Golgi recycling at an intermediate stage of cisternal maturation.bioRxiv : the preprint server for biology · 2025Article
- Biocatalytic reductive amination with CRISPR-Cas9 engineered yeast.Scientific reports · 2025Article
- ER-to-Golgi trafficking through a dynamic intermediate cis-Golgi tubular network in Arabidopsis.Nature cell biology · 2025Article
- The secretory pathway in Tetrahymena is organized for efficient constitutive secretion at ciliary pockets.iScience · 2024Article
- α-Synuclein liquid condensates fuel fibrillar α-synuclein growth.Science advances · 2023Article
- Genetically encoded fluorescent tools: Shining a little light on ER-to-Golgi transport.Free radical biology & medicine · 2022Review
- Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling.The Journal of cell biology · 2022Article
- ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.Molecular biology of the cell · 2020Article
- Structure-guided approaches to targeting stress responses in human fungal pathogens.The Journal of biological chemistry · 2020Review
- Article
- FKBP12 dimerization mutations effect FK506 binding and differentially alter calcineurin inhibition in the human pathogen Aspergillus fumigatus.Biochemical and biophysical research communications · 2020Article
- Visualizing Secretory Cargo Transport in Budding Yeast.Current protocols in cell biology · 2019Article
- Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.The Journal of cell biology · 2019Article
- Budding Yeast Has a Minimal Endomembrane System.Developmental cell · 2018Article
- FKBP Ligands-Where We Are and Where to Go?Frontiers in pharmacology · 2018Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
FK506-binding protein (FKBP) is a monomer that binds to FK506, rapamycin, and related ligands. The F36M substitution, in which Phe36 in the ligand-binding pocket is changed to Met, leads to formation of antiparallel FKBP dimers, which can be dissociated into monomers by ligand binding. This FKBP(M) mutant has been employed in the mammalian secretory pathway to generate aggregates that can be dissolved by ligand addition to create cargo waves. However, when testing this approach in yeast, we found that dissolution of FKBP(M) aggregates was inefficient. An improved reversibly dimerizing FKBP formed aggregates that dissolved more readily. This FKBP(L,V) mutant carries the F36L mutation, which increases the affinity of ligand binding, and the I90V mutation, which accelerates ligand-induced dissociation of the dimers. The FKBP(L,V) mutant expands the utility of reversibly dimerizing FKBP.
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