ArticleJournal of cell science2016
Drosophila vinculin is more harmful when hyperactive than absent, and can circumvent integrin to form adhesion complexes.
Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- The mechanical response of vinculin.Science advances · 2025Article
- Unveiling the Spectrum of Minor Genes in Cardiomyopathies: A Narrative Review.International journal of molecular sciences · 2024Review
- The actin binding sites of talin have both distinct and complementary roles in cell-ECM adhesion.PLoS genetics · 2024Article
- Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins.Molecular biology of the cell · 2023Article
- Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins.bioRxiv : the preprint server for biology · 2023Article
- The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis.The Journal of cell biology · 2023Article
- The mechanical cell - the role of force dependencies in synchronising protein interaction networks.Journal of cell science · 2022Review
- Article
- Focal adhesion-mediated cell anchoring and migration: from in vitro to in vivo.Development (Cambridge, England) · 2022Article
- Epithelial morphogenesis in theFrontiers in cell and developmental biology · 2022Article
- An assessment of the role of vinculin loss of function variants in inherited cardiomyopathy.Human mutation · 2020Article
- Mask, a component of the Hippo pathway, is required for Drosophila eye morphogenesis.Developmental biology · 2020Article
- Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology.Molecular biology of the cell · 2020Review
- Relief of talin autoinhibition triggers a force-independent association with vinculin.The Journal of cell biology · 2020Article
- Cbl-Associated Protein CAP contributes to correct formation and robust function of the Drosophila heart tube.PloS one · 2020Article
- Recruitment of Jub by α-catenin promotes Yki activity andJournal of cell science · 2019Article
- Adhesions Assemble!-Autoinhibition as a Major Regulatory Mechanism of Integrin-Mediated Adhesion.Frontiers in molecular biosciences · 2019Review
- An alternative mode of epithelial polarity in the Drosophila midgut.PLoS biology · 2018Article
- Requirement for and polarized localization of integrin proteins during Drosophila wound closure.Molecular biology of the cell · 2018Article
- Analysis of a vinculin homolog in a sponge (phylum Porifera) reveals that vertebrate-like cell adhesions emerged early in animal evolution.The Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Vinculin is a highly conserved protein involved in cell adhesion and mechanotransduction, and both gain and loss of its activity causes defective cell behaviour. Here, we examine how altering vinculin activity perturbs integrin function within the context of Drosophila development. Whereas loss of vinculin produced relatively minor phenotypes, gain of vinculin activity, through a loss of head-tail autoinhibition, caused lethality. The minimal domain capable of inducing lethality is the talin-binding D1 domain, and this appears to require talin-binding activity, as lethality was suppressed by competition with single vinculin-binding sites from talin. Activated Drosophila vinculin triggered the formation of cytoplasmic adhesion complexes through the rod of talin, but independently of integrin. These complexes contain a subset of adhesion proteins but no longer link the membrane to actin. The negative effects of hyperactive vinculin were segregated into morphogenetic defects caused by its whole head domain and lethality caused by its D1 domain. These findings demonstrate the crucial importance of the tight control of the activity of vinculin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.