ArticlePLoS pathogens2016
An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 264 papers.
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Who cites it
264 citing papers in PubMed, 586 citations in OpenAlex.
- Identification of broadly expressed nervous system-related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys.Pest management science · 2026Article
- Harnessing next-generation molecular technologies for precision control of Tuta absoluta and Helicoverpa armigera in tomato.Functional & integrative genomics · 2026Review
- RNA Activation in Plants: Current Evidence and Molecular Insights.Plants (Basel, Switzerland) · 2026Review
- Cross-kingdom RNA in plant-microbe interactions: from molecular interactions to next-generation crop protection.Stress biology · 2026Review
- Review
- Spray-Induced Gene Silencing (SIGS) of Dual-Target Genes (Journal of fungi (Basel, Switzerland) · 2026Article
- BcMFSR Is Required for Efficient Uptake of Exogenous dsRNA and Spray-Induced Gene Silencing in Botrytis cinerea.Molecular plant pathology · 2026Article
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Article
- RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends.Molecular biology reports · 2026Review
- Envisaging the role of plant Small RNAs: A comprehensive review on antiviral defense mechanisms.Molecular biology reports · 2026Review
- Rational dsRNA design, scalable production and nanodelivery to enhance spray-induced gene silencing.Communications biology · 2026Review
- Cracking the wall: the fungal cell wall assembly protein ECM33 is a promising molecular target in powdery mildew fungi.Horticulture research · 2026Article
- RNA interference in crop protection: opportunities and challenges during the transition to commercialization.Pest management science · 2026Review
- Phyllosphere microbiome responses to spray-induced gene silencing targeting Phytophthora infestans in potato.NPJ biofilms and microbiomes · 2026Article
- Nanoclay-Mediated RNA Interference Targeting Insecticide Receptors Enhances Whitefly Mortality and Reduces Fecundity.Molecular biotechnology · 2026Article
- Sprayable dsRNA for integrated crop defense: mechanisms, delivery strategies, and translational challenges.Molecular biology reports · 2026Review
- Small RNAs as systemic signals in plant defense: mechanisms, challenges, and future directions.Molecular biology reports · 2026Review
- Domain-Targeted RNAi of VeA Reveals Its Essential Role in the Fusarium oxysporum-Pseudostellaria heterophylla Interaction.Molecular plant pathology · 2026Article
- Recent Advances in Pathogenicity and Biocontrol of PostharvestJournal of fungi (Basel, Switzerland) · 2026Review
204 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meeting the increasing food and energy demands of a growing population will require the development of ground-breaking strategies that promote sustainable plant production. Host-induced gene silencing has shown great potential for controlling pest and diseases in crop plants. However, while delivery of inhibitory noncoding double-stranded (ds)RNA by transgenic expression is a promising concept, it requires the generation of transgenic crop plants which may cause substantial delay for application strategies depending on the transformability and genetic stability of the crop plant species. Using the agronomically important barley-Fusarium graminearum pathosystem, we alternatively demonstrate that a spray application of a long noncoding dsRNA (791 nt CYP3-dsRNA), which targets the three fungal cytochrome P450 lanosterol C-14α-demethylases, required for biosynthesis of fungal ergosterol, inhibits fungal growth in the directly sprayed (local) as well as the non-sprayed (distal) parts of detached leaves. Unexpectedly, efficient spray-induced control of fungal infections in the distal tissue involved passage of CYP3-dsRNA via the plant vascular system and processing into small interfering (si)RNAs by fungal DICER-LIKE 1 (FgDCL-1) after uptake by the pathogen. We discuss important consequences of this new finding on future RNA-based disease control strategies. Given the ease of design, high specificity, and applicability to diverse pathogens, the use of target-specific dsRNA as an anti-fungal agent offers unprecedented potential as a new plant protection strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.