ArticlePloS one2016
The TreadWheel: A Novel Apparatus to Measure Genetic Variation in Response to Gently Induced Exercise for Drosophila.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Gut Metabolism Links Precision Nutrition, Exercise, and Healthspan in Drosophila melanogaster.Aging cell · 2026Article
- Effect of Aerobic Exercise and a Diet Supplementation with Linoleic Acid on Metabolic Parameters inBiology · 2026Article
- The impact of exercise on protein levels in Drosophila melanogaster.Biology open · 2026Article
- Fine-tuning striated muscle performance: conserved sarcomere-level mechanisms across insect and vertebrate systems.Frontiers in physiology · 2026Review
- Gut metabolism links precision nutrition, exercise, and healthspan inbioRxiv : the preprint server for biology · 2025Article
- The role of genetic variation in shaping phenotypic responses to diet in aging Drosophila melanogaster.Heredity · 2025Article
- A new Drosophila model of prolonged inactivity shortens lifespan and impairs muscle function.Scientific reports · 2025Article
- Unpredictable disturbance and its effects on activity behavior and lifespan in Drosophila melanogaster.Biology open · 2025Article
- Article
- The implications of exercise in Drosophila melanogaster: insights into Akt/p38 MAPK/Nrf2 pathway associated with Hsp70 regulation in redox balance maintenance.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Early life exercise training and inhibition ofAging · 2022Article
- Review
- Exercise-induced changes in climbing performance.Royal Society open science · 2021Article
- GWAS reveal a role for the central nervous system in regulating weight and weight change in response to exercise.Scientific reports · 2021Article
- Navigation of a Freely Walking Fruit Fly in Infinite Space Using a Transparent Omnidirectional Locomotion Compensator (TOLC).Sensors (Basel, Switzerland) · 2021Article
- Heat shock proteins and small nucleolar RNAs are dysregulated in a Drosophila model for feline hypertrophic cardiomyopathy.G3 (Bethesda, Md.) · 2021Article
- Influence of Lab Adapted Natural Diet and Microbiota on Life History and Metabolic Phenotype ofMicroorganisms · 2020Article
- Variation in mobility and exercise adaptations between Drosophila species.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2020Article
- Alpha- and beta-adrenergic octopamine receptors in muscle and heart are required for Drosophila exercise adaptations.PLoS genetics · 2020Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Obesity is one of the dramatic health issues affecting developed and developing nations, and exercise is a well-established intervention strategy. While exercise-by-genotype interactions have been shown in humans, overall little is known. Using the natural negative geotaxis of Drosophila melanogaster, an important model organism for the study of genetic interactions, a novel exercise machine, the TreadWheel, can be used to shed light on this interaction. The mechanism for inducing exercise with the TreadWheel is inherently gentle, thus minimizing possible confounding effects of other stressors. Using this machine, we were able to assess large cohorts of adult flies from eight genetic lines for their response to exercise after one week of training. We measured their triglyceride, glycerol, protein, glycogen, glucose content, and body weight, as well as their climbing ability and feeding behavior in response to exercise. Exercised flies showed decreased stored triglycerides, glycogen, and body weight, and increased stored protein and climbing ability. In addition to demonstrating an overall effect of TreadWheel exercise on flies, we found significant interactions of exercise with genotype, sex, or genotype-by-sex effects for most of the measured phenotypes. We also observed interaction effects between exercise, genotype, and tissue (abdomen or thorax) for metabolite profiles, and those differences can be partially linked to innate differences in the flies' persistence in maintaining activity during exercise bouts. In addition, we assessed gene expression levels for a panel of 13 genes known to be associated with respiratory fitness and found that many responded to exercise. With this study, we have established the TreadWheel as a useful tool to study the effect of exercise in flies, shown significant genotype-specific and sex-specific impacts of exercise, and have laid the ground work for more extensive studies of how genetics, sex, environment, and aging interact with exercise to influence metabolic fitness in Drosophila.
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Registered trials
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