Evidence map›Paper›PMID 27736892›Full record

ArticlePloS one2016

Leaf Transcriptome Sequencing for Identifying Genic-SSR Markers and SNP Heterozygosity in Crossbred Mango Variety 'Amrapali' (Mangifera indica L.).

Ajay Kumar Mahato, Nimisha Sharma, Akshay Singh, Manish Srivastav, Jaiprakash, Sanjay Kumar Singh, Anand Kumar Singh, Tilak Raj Sharma, Nagendra Kumar Singh

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
6.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 37 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Ajay Kumar MahatoICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.
Nimisha SharmaThe Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India.
Akshay SinghICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.
Manish SrivastavThe Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India.
JaiprakashThe Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India.
Sanjay Kumar SinghThe Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India.
Anand Kumar SinghThe Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India.
Tilak Raj SharmaICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.
Nagendra Kumar SinghICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.
Indian Agricultural Research Institute · INNational Research Centre on Plant Biotechnology · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mango (Mangifera indica L.) is called "king of fruits" due to its sweetness, richness of taste, diversity, large production volume and a variety of end usage. Despite its huge economic importance genomic resources in mango are scarce and genetics of useful horticultural traits are poorly understood. Here we generated deep coverage leaf RNA sequence data for mango parental varieties 'Neelam', 'Dashehari' and their hybrid 'Amrapali' using next generation sequencing technologies. De-novo sequence assembly generated 27,528, 20,771 and 35,182 transcripts for the three genotypes, respectively. The transcripts were further assembled into a non-redundant set of 70,057 unigenes that were used for SSR and SNP identification and annotation. Total 5,465 SSR loci were identified in 4,912 unigenes with 288 type I SSR (n ≥ 20 bp). One hundred type I SSR markers were randomly selected of which 43 yielded PCR amplicons of expected size in the first round of validation and were designated as validated genic-SSR markers. Further, 22,306 SNPs were identified by aligning high quality sequence reads of the three mango varieties to the reference unigene set, revealing significantly enhanced SNP heterozygosity in the hybrid Amrapali. The present study on leaf RNA sequencing of mango varieties and their hybrid provides useful genomic resource for genetic improvement of mango.

Indexed as

Microsatellite RepeatsPolymorphism, Single NucleotideCrosses, GeneticGene Expression ProfilingHeterozygoteMangiferaPlant LeavesQuantitative Trait LociRNA, PlantSequence Analysis, RNARNA, Plant

Identifiers

PMID27736892
PMCPMC5063295
OpenAlexW2530590089

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.