Evidence map›Paper›PMID 27736791›Full record

ArticleApplied and environmental microbiology2016

The Surface-Associated Exopolysaccharide of Bifidobacterium longum 35624 Plays an Essential Role in Dampening Host Proinflammatory Responses and Repressing Local TH17 Responses.

Elisa Schiavi, Marita Gleinser, Evelyn Molloy, David Groeger, Remo Frei, Ruth Ferstl, Noelia Rodriguez-Perez, Mario Ziegler, Ray Grant, Thomas Fintan Moriarty and 8 more

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In one paragraph

Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
87citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

87 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
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  13. Harnessing postbiotics for liver health: Emerging perspectives.World journal of gastrointestinal pharmacology and therapeutics · 2025
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  20. Complementary immunoregulatory effects ofCurrent research in microbial sciences · 2025
    Article

27 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Elisa SchiaviSwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Marita GleinserAPC Microbiome Institute and School of Microbiology, University College Cork, Cork, Ireland.
Evelyn MolloyAPC Microbiome Institute and School of Microbiology, University College Cork, Cork, Ireland.
David GroegerAlimentary Health Pharma Davos, Davos, Switzerland.
Remo FreiSwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Ruth FerstlSwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Noelia Rodriguez-PerezSwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Mario ZieglerSwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Ray GrantAlimentary Health Pharma Davos, Davos, Switzerland.
Thomas Fintan MoriartyAO Research Institute Davos, Davos, Switzerland.
Stephan PlattnerAlimentary Health, Cork, Ireland.
Selena HealyAlimentary Health, Cork, Ireland.
Mary O'Connell MotherwayAPC Microbiome Institute and School of Microbiology, University College Cork, Cork, Ireland.
Cezmi A AkdisSwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Jennifer RoperAlimentary Health, Cork, Ireland.
Friedrich AltmannBOKU, Vienna, Austria.
Douwe van SinderenAPC Microbiome Institute and School of Microbiology, University College Cork, Cork, Ireland.
Liam O'MahonySwiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland liam.omahony@siaf.uzh.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The immune-modulating properties of certain bifidobacterial strains, such as Bifidobacterium longum subsp. longum 35624 (B. longum 35624), have been well described, although the strain-specific molecular characteristics associated with such immune-regulatory activity are not well defined. It has previously been demonstrated that B. longum 35624 produces a cell surface exopolysaccharide (sEPS), and in this study, we investigated the role played by this exopolysaccharide in influencing the host immune response. B. longum 35624 induced relatively low levels of cytokine secretion from human dendritic cells, whereas an isogenic exopolysaccharide-negative mutant derivative (termed sEPS IMPORTANCE: Particular gut commensals, such as B. longum 35624, are known to contribute positively to the development of mucosal immune cells, resulting in protection from inflammatory diseases. However, the molecular basis and mechanisms for these commensal-host interactions are poorly described. In this report, an exopolysaccharide was shown to be decisive in influencing the immune response to the bacterium. We generated an isogenic mutant unable to produce exopolysaccharide and observed that this mutation caused a dramatic change in the response of human immune cells in vitro In addition, the use of mouse models confirmed that lack of exopolysaccharide production induces inflammatory responses to the bacterium. These results implicate the surface-associated exopolysaccharide of the B. longum 35624 cell envelope in the prevention of aberrant inflammatory responses.

Indexed as

AnimalsBifidobacteriales InfectionsBifidobacterium longumCytokinesFemaleHumansInterleukin-17MiceMice, Inbred BALB CPolysaccharides, BacterialTh17 CellsCytokinesInterleukin-17Polysaccharides, Bacterial

Identifiers

PMID27736791
PMCPMC5118929

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.