ArticleVeterinary world2016
Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle.
Article in Veterinary world, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Investigation of Biomarkers Secreted from Adipose Tissue in Cattle Infected withVeterinary sciences · 2026Article
- Molecular epidemiology of bovine theileriosis and genetic diversity ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Multiplex PCR for rapid differential diagnosis of co-prevalent species of Theileria (Theileria annulata and Theileria orientalis) in cattle.Parasitology research · 2023Article
- A Multi-copy Nucleic Acid-Based Diagnostic Test for Bovine Tropical Theileriosis.Acta parasitologica · 2022Article
- Babesiosis and Theileriosis in North America.Pathogens (Basel, Switzerland) · 2022Review
- End Point Multiplex PCR for Diagnosis of Haemoprotozoan Diseases in Cattle.Acta parasitologica · 2021Article
- An appraisal of oriental theileriosis and the Theileria orientalis complex, with an emphasis on diagnosis and genetic characterisation.Parasitology research · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThis study aims to develop and to standardize a polymerase chain reaction (PCR) assay that will diagnose clinical as well as carrier state of the disease and to compare the results with conventional microscopy technique. MATERIALS AND
methodsA herd of crossbred cattle with the previous history of theileriosis in village Lahli, district Rohtak, Haryana, was selected for this study. A total of 29 blood samples were collected randomly from cows including five clinically ill cattle. Blood smears from all animals and lymph node biopsy smears from animal with swollen lymph nodes were examined microscopically after conventional Giemsa staining. Phenol chloroform isoamyl alcohol method was used for extracting DNA from blood. Previously published primers targeting
resultsOut of 29 samples tested,18 (62.06%) were found positive for theileriosis by PCR assay, whereas only 10 (34.48%) samples were detected positive by conventional microscopic technique using Giemsa staining method.
conclusionsOn the basis results of comparative studies, it can be concluded that PCR assay is a more sensitive than microscopic examination for detection of theileriosis. This can be attributed to the ability of PCR assay to detect small amounts of genomic DNA of
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