ArticleVeterinary world2016
Semi-quantitative digital analysis of polymerase chain reaction-electrophoresis gel: Potential applications in low-income veterinary laboratories.
Article in Veterinary world, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- Scalable human neuronal models of tauopathy producing endogenous seed-competent 4R tau.Science advances · 2026Article
- Insights into germination, physiological, and molecular changes in aniseeds induced by magnetic fields.Scientific reports · 2026Article
- Prophylactic and Therapeutic Efficacy of Ultrasonicated Rosmarinus officinalis Ethanolic Extract and its Chitosan-Loaded Nanoparticles Against Eimeria tenella Infected Broiler Chickens.Acta parasitologica · 2024Article
- A convenient analytic method for gel quantification using ImageJ paired with Python or R.PloS one · 2024Article
- Peripheral mRNA Expression and Prognostic Significance of Emotional Stress Biomarkers in Metastatic Breast Cancer Patients.International journal of molecular sciences · 2022Article
- The Assembly of Flagella in EnteropathogenicInternational journal of molecular sciences · 2022Article
- Rapid Detection ofInternational journal of molecular sciences · 2022Article
- Prevalence and molecular detection of Eimeria species in different types of poultry in Greece and associated risk factors.Parasitology research · 2022Article
- Recapitulation of endogenous 4R tau expression and formation of insoluble tau in directly reprogrammed human neurons.Cell stem cell · 2022Article
- Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation.Molecules (Basel, Switzerland) · 2020Review
- Erwinia amylovora catalases KatA and KatG are virulence factors and delay the starvation-induced viable but non-culturable (VBNC) response.Molecular plant pathology · 2018Article
- Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique.Pakistan journal of medical sciencesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThe interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a readily accessible technology in laboratories across developing nations. The aim of this study was to evaluate a semi-quantitative method for conventional PCR amplicons using digital image analysis of electrophoretic gel. The potential applications are also discussed. MATERIALS AND
methodsThis study describes standard conditions for the digital image analysis of PCR amplicons using the freely available ImageJ software and confirmed using the qPCR assay. RESULTS AND
conclusionComparison of ImageJ analysis of PCR-electrophoresis gel and qPCR methods showed similar trends in the
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.