ArticleStem cells and development2016
Prediction of Differentiation Tendency Toward Hepatocytes from Gene Expression in Undifferentiated Human Pluripotent Stem Cells.
Article in Stem cells and development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Neural network approaches, including use of topological data analysis, enhance classification of human induced pluripotent stem cell colonies by treatment condition.PLoS computational biology · 2025Article
- Secondary Publication: Proposal for Points of Consideration for Pluripotent Stem Cell Culture.In vitro cellular & developmental biology. Animal · 2024Review
- Trisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells.In vitro cellular & developmental biology. Animal · 2024Article
- A live-cell image-based machine learning strategy for reducing variability in PSC differentiation systems.Cell discovery · 2023Article
- Sustained intrinsic WNT and BMP4 activation impairs hESC differentiation to definitive endoderm and drives the cells towards extra-embryonic mesoderm.Scientific reports · 2021Article
- Gene Editing Correction of a Urea Cycle Defect in Organoid Stem Cell Derived Hepatocyte-like Cells.International journal of molecular sciences · 2021Article
- Practical choice for robust and efficient differentiation of human pluripotent stem cells.World journal of stem cells · 2020Review
- Cytotoxicity assay using a human pluripotent stem cell-derived cranial neural crest cell model.In vitro cellular & developmental biology. Animal · 2020Article
- Predicting differentiation potential of human pluripotent stem cells: Possibilities and challenges.World journal of stem cells · 2019Article
- Biliary atresia combined with progressive familial intrahepatic cholestasis type 3: A case report and review of the literature.Medicine · 2019Review
- Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array.Journal of visualized experiments : JoVE · 2018Article
- Decellularized human liver extracellular matrix (hDLM)-mediated hepatic differentiation of human induced pluripotent stem cells (hIPSCs).Journal of tissue engineering and regenerative medicine · 2018Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional hepatocytes derived from human pluripotent stem cells (hPSCs) have potential as tools for predicting drug-induced hepatotoxicity in the early phases of drug development. However, the propensity of hPSC lines to differentiate into specific lineages is reported to differ. The ability to predict low propensity of hPSCs to differentiate into hepatocytes would facilitate the selection of useful hPSC clones and substantially accelerate development of hPSC-derived hepatocytes for pharmaceutical research. In this study, we compared the expression of genes associated with hepatic differentiation in five hPSC lines including human ES cell line, H9, which is known to differentiate into hepatocytes, and an hPSC line reported with a poor propensity for hepatic differentiation. Genes distinguishing between undifferentiated hPSCs, hPSC-derived hepatoblast-like differentiated cells, and primary human hepatocytes were drawn by two-way cluster analysis. The order of expression levels of genes in undifferentiated hPSCs was compared with that in hPSC-derived hepatoblast-like cells. Three genes were selected as predictors of low propensity for hepatic differentiation. Expression of these genes was investigated in 23 hPSC clones. Review of representative cells by induction of hepatic differentiation suggested that low prediction scores were linked with low hepatic differentiation. Thus, our model using gene expression ranking and bioinformatic analysis could reasonably predict poor differentiation propensity of hPSC lines.
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