ArticlePLoS pathogens2016
Combined Activity of DCL2 and DCL3 Is Crucial in the Defense against Potato Spindle Tuber Viroid.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Engineering the 3'-UTR of Tobacco Vein Mottling Virus to Confer Cross-Protection Against Potyviruses.Molecular plant pathology · 2026Article
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- RNA Interference in Plant Interactions with Pathogenic Microorganisms: A Weapon or a Liability?Current issues in molecular biology · 2025Review
- Derailing the host machinery to achieve replication: how viroid and viroid-like RNAs successfully copy their genomes in hostile territory.RNA biology · 2025Review
- Evolutionarily New Solanaceous DCL2 Family Members Acquire Functions in Tomato.Molecular plant pathology · 2025Article
- Hop Stunt Viroid Expression and Host Responses in Arabidopsis thaliana.Molecular plant pathology · 2025Article
- Stress-related transcriptomic changes associated with GFP transgene expression and active transgene silencing in plants.Scientific reports · 2024Article
- Evidence for an RNAi-independent role of Arabidopsis DICER-LIKE2 in growth inhibition and basal antiviral resistance.The Plant cell · 2024Article
- Review
- Genome-wide identification and in silico characterization of major RNAi gene families in date palm (Phoenix dactylifera).BMC genomic data · 2024Article
- Knockout of SlDCL2b attenuates the resistance of tomato to potato spindle tuber viroid infection.Molecular plant pathology · 2024Article
- Article
- Genome-Wide Identification and Characterization of Major RNAi Genes Highlighting Their Associated Factors in Cowpea (BioMed research international · 2023Article
- PSTVd infection inFrontiers in plant science · 2023Article
- Emerging value of the viroid model in molecular biology and beyond.Virus research · 2022Review
- RNAi-Based Antiviral Innate Immunity in Plants.Viruses · 2022Review
- Article
- Genetic evidence for the involvement of Dicer-like 2 and 4 as well as Argonaute 2 in theThe Journal of general virology · 2021Article
- RNA-dependent RNA polymerase 1 delays the accumulation of viroids in infected plants.Molecular plant pathology · 2021Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viroids are self replicating non-coding RNAs capable of infecting a wide range of plant hosts. They do not encode any proteins, thus the mechanism by which they escape plant defenses remains unclear. RNAi silencing is a major defense mechanism against virus infections, with the four DCL proteins being principal components of the pathway. We have used Nicotiana benthamiana as a model to study Potato spindle tuber viroid infection. This viroid is a member of the Pospiviroidae family and replicates in the nucleus via an asymmetric rolling circle mechanism. We have created knock-down plants for all four DCL genes and their combinations. Previously, we showed that DCL4 has a positive effect on PSTVd infectivity since viroid levels drop when DCL4 is suppressed. Here, we show that PSTVd levels remain decreased throughout infection in DCL4 knockdown plants, and that simultaneous knockdown of DCL1, DCL2 or DCL3 together with DCL4 cannot reverse this effect. Through infection of plants suppressed for multiple DCLs we further show that a combined suppression of DCL2 and DCL3 has a major effect in succumbing plant antiviral defense. Based on our results, we further suggest that Pospoviroids may have evolved to be primarily processed by DCL4 as it seems to be a DCL protein with less detrimental effects on viroid infectivity. These findings pave the way to delineate the complexity of the relationship between viroids and plant RNA silencing response.
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