ArticlePloS one2016
A Rapid and Simple Method for Microscopy-Based Stomata Analyses.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Effects of Atmospheric COPlant, cell & environment · 2026Article
- Disruption of SlCNGC18 enhances Cucumber mosaic virus resistance and drought tolerance in tomato (Solanum lycopersicum L. cv. Micro-Tom).BMC plant biology · 2026Article
- Genetic markers of stomatal cluster development in Begoniaceae revealed through trait analysis assisted by interactive deep-learning.Plant physiology · 2026Article
- Drought stress inFrontiers in plant science · 2026Review
- Advanced phenotyping features utilizing deep learning techniques for automated analysis of stomatal guard cell orientation.Scientific reports · 2025Article
- Salmonella enterica exploits the auxin signaling pathway to overcome stomatal immunity.PLoS pathogens · 2025Article
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- Arabidopsis COP1 guides stomatal response in guard cells through pH regulation.Communications biology · 2024Article
- LncRNA DANA1 promotes drought tolerance and histone deacetylation of drought responsive genes in Arabidopsis.EMBO reports · 2024Article
- An optimization method for measuring the stomata in cassava (Open life sciences · 2024Article
- Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance.Plant physiology · 2023Article
- Cytokinin Deficiency Alters Leaf Proteome and Metabolome during Effector-Triggered Immunity inPlants (Basel, Switzerland) · 2022Article
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- Evolutionarily conserved bacterial effectors hijack abscisic acid signaling to induce an aqueous environment in the apoplast.Cell host & microbe · 2022Article
- The DnaJ-like Zinc Finger Protein ORANGE Promotes Proline Biosynthesis in Drought-StressedInternational journal of molecular sciences · 2022Article
- StomataScorer: a portable and high-throughput leaf stomata trait scorer combined with deep learning and an improved CV model.Plant biotechnology journal · 2022Article
- Characterizing stomatal attributes and photosynthetic induction in relation to biochemical changes inFrontiers in plant science · 2022Article
- HD2A and HD2C co-regulate drought stress response by modulating stomatal closure and root growth in Arabidopsis.Frontiers in plant science · 2022Article
- Optimizing the Experimental Method for Stomata-Profiling Automation of Soybean Leaves Based on Deep Learning.Plants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are two major methodical approaches with which changes of status in stomatal pores are addressed: indirectly by measurement of leaf transpiration, and directly by measurement of stomatal apertures. Application of the former method requires special equipment, whereas microscopic images are utilized for the direct measurements. Due to obscure visualization of cell boundaries in intact leaves, a certain degree of invasive leaf manipulation is often required. Our aim was to develop a protocol based on the minimization of leaf manipulation and the reduction of analysis completion time, while still producing consistent results. We applied rhodamine 6G staining of Arabidopsis thaliana leaves for stomata visualization, which greatly simplifies the measurement of stomatal apertures. By using this staining protocol, we successfully conducted analyses of stomatal responses in Arabidopsis leaves to both closure and opening stimuli. We performed long-term monitoring of living stomata and were able to document the same leaf before and after treatment. Moreover, we developed a protocol for rapid-fixation of epidermal peels, which enables high throughput data analysis. The described method allows analysis of stomatal apertures with minimal leaf manipulation and usage of the same leaf for sequential measurements, and will facilitate the analysis of several lines in parallel.
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Registered trials
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