Evidence map›Paper›PMID 27732087›Full record

ArticleEndocrinology2016

AMPKα2 in Kiss1 Neurons Is Required for Reproductive Adaptations to Acute Metabolic Challenges in Adult Female Mice.

Marcio A Torsoni, Beatriz C Borges, Jessica L Cote, Susan J Allen, Erica Mahany, David Garcia-Galiano, Carol F Elias

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
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  4. Review
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  8. Review
  9. Article
  10. Review
  11. Article
  12. Roles of AMP-Activated Protein Kinase (AMPK) in Mammalian Reproduction.Frontiers in cell and developmental biology · 2020
    Review
  13. Metabolic regulation of female puberty via hypothalamic AMPK-kisspeptin signaling.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  14. Review
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Marcio A TorsoniLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
Beatriz C BorgesLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
Jessica L CoteLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
Susan J AllenLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
Erica MahanyLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
David Garcia-GalianoLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
Carol F EliasLaboratory of Metabolism Disorders (M.A.T.), School of Applied Sciences, State University of Campinas, Limeira-SP 13484-350, Brazil; and Department of Molecular and Integrative Physiology (M.A.T., B.C.B., S.J.A., D.G.-G., C.F.E.), Neuroscience Graduate Program (J.L.C.), and Department of Obstetrics and Gynecology (E.M., C.F.E.), University of Michigan, Ann Arbor, Michigan 48109.
University of Michigan–Ann Arbor · USUniversidade Estadual de Campinas (UNICAMP) · BR

Funding

Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Mehboob A Hussain · 2013 to 2026
$24.3M
Testosterone-GnRH Frequency and the Evolution of PCOS in AdolescenceP50HD028934 · NICHD · UNIVERSITY OF VIRGINIA · PI HAISENLEDER, DANIEL J. · 2014 to 2018
$8.6M
Neural basis of leptin action on reproductionR01HD069702 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI ELIAS, CAROL FUZETI · 2012 to 2021
$3.4M
NICHD NIH HHS P50 HD028934NICHD NIH HHS R01 HD069702NIDDK NIH HHS P30 DK020572
6 · The paper itself

Abstract

A temporary and reversible inhibition of the hypothalamo-pituitary-gonadal axis is adaptive when energy reserves are diminished, allowing individual survival and energy accumulation for eventual reproduction. The AMP-activated protein kinase (AMPK) works as a cellular sensor of the AMP to ATP ratio and ultimately of energy availability. Activation of AMPK suppresses ATP-consuming processes and stimulates ATP-producing pathways. The AMPK α2 catalytic subunit is expressed in multiple hypothalamic nuclei including those associated with reproductive control, ie, the anteroventral periventricular nucleus and the arcuate nucleus. Subsets of kisspeptin neurons in the anteroventral periventricular nucleus (20% in females) and arcuate nucleus (45% in males and 65% in females) coexpress AMPKα2 mRNA. Using the Cre-loxP approach, we assessed whether AMPKα2 in Kiss1 cells is required for body weight and reproductive function. The AMPKα2-deleted mice show no difference in body weight and time for sexual maturation compared with controls. Males and females are fertile and have normal litter size. The AMPKα2-deleted and control females have similar estradiol feedback responses and show no difference in Kiss1 mRNA expression after ovariectomy or ovariectomy plus estradiol replacement. In males, acute fasting decreased Kiss1 mRNA expression in both groups, but no effect was observed in females. However, after an acute fasting, control mice displayed prolonged diestrous phase, but AMPKα2-deleted females showed no disruption of estrous cycles. Our findings demonstrate that the AMPKα2 catalytic subunit in Kiss1 cells is dispensable for body weight and reproductive function in mice but is necessary for the reproductive adaptations to conditions of acute metabolic distress.

Indexed as

AMP-Activated Protein KinasesAnimalsBody WeightEstrous CycleFastingFemaleHypothalamusKisspeptinsMaleMiceMice, KnockoutNeuronsSexual MaturationAMP-Activated Protein KinasesKiss1 protein, mouseKisspeptinsPrkaa2 protein, mouse

Identifiers

PMID27732087
PMCPMC5133340
OpenAlexW2531279040

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.