ArticleScientific reports2016
Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Tissue Context Shapes N-Glycan Processing and Site-Specific Microheterogeneity in Adult Drosophila melanogaster Compared With S2 Cells.Archives of insect biochemistry and physiology · 2026Article
- First site-specific N-glycoproteome of the spider mite Tetranychus urticae reveals a glycan signature different from insect models and functional insights for pest management.Experimental & applied acarology · 2026Article
- N-Glycoengineering of insect cells for tri-antennary N-glycan biosynthesis.Scientific reports · 2026Article
- Article
- Genome architecture and selective signals compensatorily shape plastic response to a new environment.Innovation (Cambridge (Mass.)) · 2023Article
- Interactions between Entomopathogenic Fungi and Insects and Prospects with Glycans.Journal of fungi (Basel, Switzerland) · 2023Review
- Increasing Complexity of the N-Glycome During Caenorhabditis Development.Molecular & cellular proteomics : MCP · 2023Article
- 35 years in plant lectin research: a journey from basic science to applications in agriculture and medicine.Glycoconjugate journal · 2022Article
- Mosquito-larvicidal Binary (BinA/B) proteins for mosquito control programs -advancements, challenges, and possibilities.Current research in insect science · 2022Review
- Extracellular matrix protein N-glycosylation mediates immune self-tolerance inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Identification of N-linked Glycoproteins in Silkworm Serum Using Con A Lectin Affinity Chromatography and Mass Spectrometry.Journal of insect science (Online) · 2021Article
- RNAi-Mediated Silencing of Pgants Shows Core 1Frontiers in physiology · 2021Article
- Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.Molecular omics · 2020Article
- N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Molecular & cellular proteomics : MCP · 2020Article
- Let's talk about sexes: sex-related N-glycosylation in ecologically important invertebrates.Glycoconjugate journal · 2020Review
- Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick,Vaccines · 2020Article
- Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.Molecular & cellular proteomics : MCP · 2018Article
- Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines.In vitro cellular & developmental biology. Animal · 2017Article
- Distribution of Glycan Motifs at the Surface of Midgut Cells in the Cotton Leafworm (Frontiers in physiology · 2017Article
- N-glycosylation in Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biologyArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on N-glycosylated proteins. N-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. Here we used the red flour beetle, Tribolium castaneum, to perform a first comprehensive study on the involvement of the protein N-glycosylation pathway in metamorphosis. The transcript levels for genes encoding N-glycan processing enzymes increased during later developmental stages and, in turn, transition from larva to adult coincided with an enrichment of more extensively modified paucimannose glycans, including fucosylated ones. Blockage of N-glycan attachment resulted in larval mortality, while RNAi of α-glucosidases involved in early N-glycan trimming and quality control disrupted the larva to pupa transition. Additionally, simultaneous knockdown of multiple genes responsible for N-glycan processing towards paucimannose structures revealed their novel roles in pupal appendage formation and adult eclosion. Our findings revealed that, next to hormonal control, insect post-embryonic development and metamorphosis depend on protein N-glycan attachment and efficient N-glycan processing. Consequently, disruption of these processes could be an effective new approach for insect control.
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