ArticleVirus genes2017
Construction of a full-length infectious cDNA clone of Cowpea mild mottle virus.
Article in Virus genes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Reverse genetics platform for tomato mottle mosaic virus: infectious clone construction and resistance screening in tomato.Molecular biology reports · 2026Article
- Construction and Characterization of a Botrytis Virus F Infectious Clone.Journal of fungi (Basel, Switzerland) · 2022Article
- Transient reprogramming of crop plants for agronomic performance.Nature plants · 2021Article
- Article
- Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.Plant biotechnology journal · 2019Review
- Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks.Virology journal · 2019Article
- Construction of an agroinfectious clone of bean rugose mosaic virus using Gibson Assembly.Virus genes · 2017Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious cDNA clones are an important tool to study the molecular and cellular process of RNA virus infection. In vitro and in vivo transcription systems are the two main strategies used in the generation of infectious cDNA clones for RNA viruses. This study describes the first generation of a full-length infectious cDNA clone of Cowpea mild mottle virus (CPMMV), a Carlavirus. The full-length genome was synthesized by Overlap Extension PCR of two overlapping fragments and cloned in a pUC-based vector under control of the SP6 RNA polymerase promoter. After in vitro run-off transcription, the produced RNA was mechanically inoculated into soybean plants cv. CD206. The systemic infection was confirmed by RT-PCR and further sequencing of amplified cDNA fragments. To simplify the transfection process, the complete genome was subcloned into a binary vector under control of the 35S promoter of cauliflower mosaic virus by the Gibson Assembly protocol. The resulting clones were inoculated by particle bombardment onto soybean seedlings and the recovery of the virus was confirmed 2 weeks later by RT-PCR. Our results indicate the constructs of the full-length cDNA of CPMMV are fully infectious in both in vitro and in vivo transcription strategies.
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