ArticleProceedings of the National Academy of Sciences of the United States of America2016
Working stroke of the kinesin-14, ncd, comprises two substeps of different direction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- What Determines the Directionality of Kinesins? Studying the Anomalous Directionality of Nonclaret Disjunctional Motor Mutants.Journal of the American Chemical Society · 2026Article
- Bound ion effects: Using machine learning method to study the kinesin Ncd's binding with microtubule.Biophysical journal · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.The Journal of cell biology · 2024Article
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Human kinesin-5 KIF11 drives the helical motion of anti-parallel and parallel microtubules around each other.The EMBO journal · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.bioRxiv : the preprint server for biology · 2023Article
- Membrane-bound myosin IC drives the chiral rotation of the gliding actin filament around its longitudinal axis.Scientific reports · 2023Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Motor generated torque drives coupled yawing and orbital rotations of kinesin coated gold nanorods.Communications biology · 2022Article
- Anchoring geometry is a significant factor in determining the direction of kinesin-14 motility on microtubules.Scientific reports · 2022Article
- Active beating modes of two clamped filaments driven by molecular motors.Journal of the Royal Society, Interface · 2022Article
- Opposing motors provide mechanical and functional robustness in the human spindle.Developmental cell · 2021Article
- CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.Communications biology · 2021Article
- IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.EMBO reports · 2020Article
- Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other.Nature communications · 2020Article
- An estimate to the first approximation of microtubule rupture force.European biophysics journal : EBJ · 2019Article
- A Brownian Ratchet Model Explains the Biased Sidestepping of Single-Headed Kinesin-3 KIF1A.Biophysical journal · 2019Article
- An automated in vitro motility assay for high-throughput studies of molecular motors.Lab on a chip · 2018Article
- Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules.Nature communications · 2018Article
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Authors and funding
6 authors.
Funding
Abstract
Single-molecule experiments have been used with great success to explore the mechanochemical cycles of processive motor proteins such as kinesin-1, but it has proven difficult to apply these approaches to nonprocessive motors. Therefore, the mechanochemical cycle of kinesin-14 (ncd) is still under debate. Here, we use the readout from the collective activity of multiple motors to derive information about the mechanochemical cycle of individual ncd motors. In gliding motility assays we performed 3D imaging based on fluorescence interference contrast microscopy combined with nanometer tracking to simultaneously study the translation and rotation of microtubules. Microtubules gliding on ncd-coated surfaces rotated around their longitudinal axes in an [ATP]- and [ADP]-dependent manner. Combined with a simple mechanical model, these observations suggest that the working stroke of ncd consists of an initial small movement of its stalk in a lateral direction when ADP is released and a second, main component of the working stroke, in a longitudinal direction upon ATP binding.
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