ArticleCirculation research2017
SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
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Article in Circulation research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 100 citations in OpenAlex.
- Local calcium dynamics and signalling in cardiomyocytes.Nature reviews. Cardiology · 2026Review
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- The Landscape of SERCA2 in Cardiovascular Diseases: Expression Regulation, Therapeutic Applications, and Emerging Roles.Biomolecules · 2026Review
- Cyclic AMP-dependent regulation of ryanodine receptors in healthy and diseased hearts.Journal of molecular and cellular cardiology · 2026Review
- Cardiac Cell Remodeling in Obesity and Diabetes.Handbook of experimental pharmacology · 2026Review
- Phosphorylation of RYR1 at SerScience signaling · 2025Article
- Mechanisms underlying dilated cardiomyopathy associated with FKBP12 deficiency.The Journal of general physiology · 2025Article
- Targeting calpain-2-mediated junctophilin-2 cleavage delays heart failure progression following myocardial infarction.Journal of molecular and cellular cardiology · 2024Article
- Rare Variant inCirculation. Genomic and precision medicine · 2024Article
- The cardiac blood transcriptome predicts de novo onset of atrial fibrillation in heart failure.Journal of molecular and cellular cardiology plus · 2024Article
- Integrated multi-omics approach reveals the role of striated muscle preferentially expressed protein kinase in skeletal muscle including its relationship with myospryn complex.Journal of cachexia, sarcopenia and muscle · 2024Article
- Structure, Function, and Regulation of the Junctophilin Family.Annual review of physiology · 2024Review
- Striated preferentially expressed gene deficiency leads to mitochondrial dysfunction in developing cardiomyocytes.Basic research in cardiology · 2024Article
- Long-term efficacy and safety of cardiac genome editing for catecholaminergic polymorphic ventricular tachycardia.The journal of cardiovascular aging · 2024Article
- The predictive value of peripheral blood cell mitochondrial gene expression in identifying the prognosis in pediatric sepsis at preschool age.Frontiers in cellular and infection microbiology · 2024Article
- Molecular Insights into the MLCK Activation by CaM.Journal of chemical information and modeling · 2023Article
- Speg interactions that regulate the stability of excitation-contraction coupling protein complexes in triads and dyads.Communications biology · 2023Article
- Novel SPEG variants in a neonate with severe dilated cardiomyopathy and relatively mild hypotonia.Human genome variation · 2023Article
- Article
- Reduction in Junctophilin 2 Expression in Cardiac Nodal Tissue Results in Intracellular Calcium-Driven Increase in Nodal Cell Automaticity.Circulation. Arrhythmia and electrophysiology · 2023Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 1 institution in 1 country.
Ann P QuickFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Qiongling WangFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Leonne E PhilippenFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Giselle Barreto-TorresFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
David Y ChiangFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
David BeaversFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Guoliang WangFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Maha KhalidFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Julia O ReynoldsFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Hannah M CampbellFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Jordan ShowellFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Mark D McCauleyFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Arjen ScholtenFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.).
Xander H T WehrensFrom the Department of Molecular Physiology and Biophysics (A.P.Q., Q.W., L.E.P., G.B.-T., J.O.R., H.M.C., J.S., X.H.T.W), Cardiovascular Research Institute (A.P.Q., Q.W., L.E.P., G.B.-T., D.Y.C., D.B., G.W., J.O.R., H.M.C., J.S., M.D.M., X.H.T.W), Medical Scientist Training Program (D.Y.C., D.B., H.M.C.), Department of Medicine (Cardiology) (M.D.M., X.H.T.W), and Pediatrics (X.H.T.W.), Baylor College of Medicine, Houston, TX; Accelerated BS/MD Program, Department of Biology and Biochemistry, University of Houston, TX (M.K.); and Department of Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands (A.S.). wehrens@bcm.edu.
University of Houston · US
Funding
Consortium for Broad Based Disease Phenotyping of Knockout MiceU54HG006348 · NHGRI · BAYLOR COLLEGE OF MEDICINE · PI BEAUDET, ARTHUR L., PAYLOR, RICHARD E · 2011 to 2015
$17.0MMolecular Physiology of the Cardiovascular SystemT32HL007676 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI HAMILTON, SUSAN L · 1989 to 2020
$7.9MYEARS 19 - 23: BCM POST-BACCALAUREATE RESEARCH EDUCATION PROGRAMR25GM069234 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI PEREIRA, FRED A · 2003 to 2024
$7.5MRyanodine receptor regulation in post-operative atrial fibrillationR01HL089598 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI WEHRENS, XANDER H.T. · 2007 to 2022
$6.5MRegulation of Sarcoplasmic Reticulum Calcium Release in Heart FailureR01HL091947 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI WEHRENS, XANDER H.T. · 2009 to 2019
$3.2MDetermining the Role of Junctophilin-2 in Cardiac DiseaseR01HL117641 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI WEHRENS, XANDER H.T. · 2014 to 2017
$2.3MPPP1R12C contributes to myosin light chain de-phosphorylation and atrial hypocontractility in atrial fibrillationK08HL130587 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MCCAULEY, MARK D · 2016 to 2020
$892kDevelopment of novel compounds for the treatment of atrial fibrillationR41HL129570 · NHLBI · ELEX BIOTECH, LLC · PI WEHRENS, XANDER H.T. · 2015 to 2015
$428kNHGRI NIH HHS U54 HG006348NHLBI NIH HHS K08 HL130587NHLBI NIH HHS R01 HL089598NHLBI NIH HHS R01 HL091947NHLBI NIH HHS R01 HL117641NHLBI NIH HHS R41 HL129570NHLBI NIH HHS T32 HL007676NIGMS NIH HHS R25 GM069234Wellcome Trust
Abstract
rationaleJunctional membrane complexes (JMCs) in myocytes are critical microdomains, in which excitation-contraction coupling occurs. Structural and functional disruption of JMCs underlies contractile dysfunction in failing hearts. However, the role of newly identified JMC protein SPEG (striated muscle preferentially expressed protein kinase) remains unclear.
objectiveTo determine the role of SPEG in healthy and failing adult hearts. METHODS AND
resultsProteomic analysis of immunoprecipitated JMC proteins ryanodine receptor type 2 and junctophilin-2 (JPH2) followed by mass spectrometry identified the serine-threonine kinase SPEG as the only novel binding partner for both proteins. Real-time polymerase chain reaction revealed the downregulation of SPEG mRNA levels in failing human hearts. A novel cardiac myocyte-specific Speg conditional knockout (MCM-Speg
conclusionsThe novel JMC protein SPEG is downregulated in human failing hearts. Acute loss of SPEG in mouse hearts causes JPH2 dephosphorylation and transverse-tubule loss associated with downstream Ca
Indexed as
AdultAgedAnimalsFemaleHeart FailureHEK293 CellsHumansMaleMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMiddle AgedMuscle ProteinsMyocytes, CardiacMyosin-Light-Chain KinaseJP2 protein, mouseMembrane ProteinsMuscle ProteinsMyosin-Light-Chain KinaseSpeg protein, mousecalcium signalingheart failuremass spectrometryphosphorylationproteomics
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